Studies n the daily activity of Glossina longipennis at Galana Ranch using a black odour-baited electrocuting target confirmed its crepuscular activity profile. Activity started at 05.00-05.30 hours and peaked at 06.00-06.30 hours, stopped by 09.00 hours, then started again at 17.00-17.30 hours with a peak at 18.30-19.00 hours, ceasing by 19.30 hours. Females made up 60% of the overall catch, and tended to arrive later than males. Other stationary sampling methods (trap, stationary ox) gave similar results. With the stationary methods, very few flies were caught outside the periods of peak activity (only 1.5% of the total between 09.00 and 17.00 hours); the ox was the only stationary bait to catch any flies between 10.00 and 16.00 hours. More flies were caught throughout the day at mobile baits (8.3% of the male and 2.3% of the female catch was taken between 09.00 and 17.00 hours). Mobile baits caught considerably more males than females (females were 17% of the catch). These males had on average higher fat and haematin reserves. Similar nutritional differences were not observed for females. There were fewer older females (ovarian category 3 or more) in mobile compared to stationary baits, and a lower proportion of the youngest males (wing fray category 1) at natural compared with artificial baits.