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The preparation of PC with the 14C-fatty acids palmitic, stearic, oleic and linoleic acid at OH-group in position 2 is described. The starting material is egg yolk lecithin. By the attack of snake venom phospholiphase lyso-PC is produced which is reacylated by the appropriate fatty acid anhydride. In comparison with the methods published up to now this preparation has the advantage of higher yields and greater simplicity. By means of in vivo synthesis it is impossible to get PC species with only one fatty acid in a defined position. Working with radioactive fatty acids the specific radioactivity can be adapted to the requirements. The procedure can be made on a semi-technical scale.
G Schulze, and
K Jung, and
D Kunze, and
E Egger
January 1981,
Preparative biochemistry,
G Schulze, and
K Jung, and
D Kunze, and
E Egger
May 2002,
Biotechnology and bioengineering,
G Schulze, and
K Jung, and
D Kunze, and
E Egger
April 1925,
Proceedings of the National Academy of Sciences of the United States of America,
G Schulze, and
K Jung, and
D Kunze, and
E Egger
December 1968,
Analytical biochemistry,
G Schulze, and
K Jung, and
D Kunze, and
E Egger
November 2017,
Journal of oleo science,
G Schulze, and
K Jung, and
D Kunze, and
E Egger
December 2012,
Food chemistry,
G Schulze, and
K Jung, and
D Kunze, and
E Egger
September 2010,
AAPS PharmSciTech,
G Schulze, and
K Jung, and
D Kunze, and
E Egger
October 1968,
Analytical biochemistry,
G Schulze, and
K Jung, and
D Kunze, and
E Egger
January 1968,
Analytical biochemistry,
G Schulze, and
K Jung, and
D Kunze, and
E Egger
January 1973,
Biochimica et biophysica acta,
