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We tested the hypothesis that increased endothelial cell adenosine 3',5'-cyclic monophosphate (cAMP) decreases microvascular permeability in vivo. The effects of cAMP-specific phosphodiesterase type IV inhibition and adenylate cyclase activation on microvascular hydraulic conductivity (Lp) were investigated in intact individual capillaries and postcapillary venules in mesentery of pithed frogs (Rana pipiens). Treatment with rolipram (10 microM) and forskolin (5 microM) for 25 min decreased Lp to 37% of control. Rolipram alone also significantly decreased Lp. Isoproterenol (10 microM) decreased Lp to 27% of control within 20 min. A subgroup of eight vessels treated with rolipram and forskolin, in which mean Lp fell to 25% of control, was examined with transmission electron microscopy. The mean number of tight junctions in the treated vessels was 2.2 per cleft (303 clefts), significantly higher than in a matched control group (192 clefts), which was 1.7 per cleft. The results indicate that microvascular Lp can be modulated by intracellular cAMP and that one of the structural end points of stimulated cAMP levels is an increase in the mean number of tight-junction strands between endothelial cells.
R H Adamson, and
B Liu, and
G N Fry, and
L L Rubin, and
F E Curry
April 2002,
American journal of physiology. Heart and circulatory physiology,
R H Adamson, and
B Liu, and
G N Fry, and
L L Rubin, and
F E Curry
February 2013,
Cellular and molecular life sciences : CMLS,
R H Adamson, and
B Liu, and
G N Fry, and
L L Rubin, and
F E Curry
October 2021,
Ecotoxicology and environmental safety,
R H Adamson, and
B Liu, and
G N Fry, and
L L Rubin, and
F E Curry
October 2000,
Pharmaceutical science & technology today,
R H Adamson, and
B Liu, and
G N Fry, and
L L Rubin, and
F E Curry
August 2009,
American journal of physiology. Lung cellular and molecular physiology,
R H Adamson, and
B Liu, and
G N Fry, and
L L Rubin, and
F E Curry
May 2000,
Journal of cell science,
R H Adamson, and
B Liu, and
G N Fry, and
L L Rubin, and
F E Curry
December 2008,
Molecular bioSystems,
R H Adamson, and
B Liu, and
G N Fry, and
L L Rubin, and
F E Curry
November 1975,
Proceedings of the National Academy of Sciences of the United States of America,
R H Adamson, and
B Liu, and
G N Fry, and
L L Rubin, and
F E Curry
November 2003,
The Journal of infectious diseases,
R H Adamson, and
B Liu, and
G N Fry, and
L L Rubin, and
F E Curry
January 1987,
Acta physiologica et pharmacologica latinoamericana : organo de la Asociacion Latinoamericana de Ciencias Fisiologicas y de la Asociacion Latinoamericana de Farmacologia,
