BIOMAT Database Logo BIOMAT Database Logo

Delta-atracotoxins from Australian funnel-web spiders compete with scorpion alpha-toxin binding on both rat brain and insect sodium channels. 1998

M J Little, and H Wilson, and C Zappia, and S Cestèle, and M I Tyler, and M F Martin-Eauclaire, and D Gordon, and G M Nicholson
Department of Health Sciences, University of Technology, Broadway, NSW, Australia.

Atracotoxins are novel peptide toxins from the venom of Australian funnel-web spiders that slow sodium current inactivation in a similar manner to scorpion alpha-toxins. To analyse their interaction with known sodium channel neurotoxin receptor sites we determined their effect on scorpion toxin, batrachotoxin and saxitoxin binding. Nanomolar concentrations of delta-atracotoxin-Hv1 and delta-atracotoxin-Ar1 completely inhibited the binding of the scorpion alpha-toxin AaH II to rat brain synaptosomes as well as the binding of LqhalphaIT, a scorpion alpha-toxin highly active on insects, to cockroach neuronal membranes. Moreover, delta-atracotoxin-Hv1 cooperatively enhanced batrachotoxin binding to rat brain synaptosomes in an analogous fashion to scorpion alpha-toxins. Thus the delta-atracotoxins represent a new class of toxins which bind to both mammalian and insect sodium channels at sites similar to, or partially overlapping with, the receptor binding sites of scorpion alpha-toxins.

UI MeSH Term Description Entries
D007313 Insecta Members of the phylum ARTHROPODA composed or organisms characterized by division into three parts: head, thorax, and abdomen. They are the dominant group of animals on earth with several hundred thousand different kinds. Three orders, HEMIPTERA; DIPTERA; and SIPHONAPTERA; are of medical interest in that they cause disease in humans and animals. (From Borror et al., An Introduction to the Study of Insects, 4th ed, p1). Insects,Insect
D007457 Iodine Radioisotopes Unstable isotopes of iodine that decay or disintegrate emitting radiation. I atoms with atomic weights 117-139, except I 127, are radioactive iodine isotopes. Radioisotopes, Iodine
D008297 Male Males
D008810 Mice, Inbred C57BL One of the first INBRED MOUSE STRAINS to be sequenced. This strain is commonly used as genetic background for transgenic mouse models. Refractory to many tumors, this strain is also preferred model for studying role of genetic variations in development of diseases. Mice, C57BL,Mouse, C57BL,Mouse, Inbred C57BL,C57BL Mice,C57BL Mice, Inbred,C57BL Mouse,C57BL Mouse, Inbred,Inbred C57BL Mice,Inbred C57BL Mouse
D008969 Molecular Sequence Data Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories. Sequence Data, Molecular,Molecular Sequencing Data,Data, Molecular Sequence,Data, Molecular Sequencing,Sequencing Data, Molecular
D009498 Neurotoxins Toxic substances from microorganisms, plants or animals that interfere with the functions of the nervous system. Most venoms contain neurotoxic substances. Myotoxins are included in this concept. Alpha-Neurotoxin,Excitatory Neurotoxin,Excitotoxins,Myotoxin,Myotoxins,Neurotoxin,Alpha-Neurotoxins,Excitatory Neurotoxins,Excitotoxin,Alpha Neurotoxin,Alpha Neurotoxins,Neurotoxin, Excitatory,Neurotoxins, Excitatory
D001921 Brain The part of CENTRAL NERVOUS SYSTEM that is contained within the skull (CRANIUM). Arising from the NEURAL TUBE, the embryonic brain is comprised of three major parts including PROSENCEPHALON (the forebrain); MESENCEPHALON (the midbrain); and RHOMBENCEPHALON (the hindbrain). The developed brain consists of CEREBRUM; CEREBELLUM; and other structures in the BRAIN STEM. Encephalon
D002462 Cell Membrane The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells. Plasma Membrane,Cytoplasmic Membrane,Cell Membranes,Cytoplasmic Membranes,Membrane, Cell,Membrane, Cytoplasmic,Membrane, Plasma,Membranes, Cell,Membranes, Cytoplasmic,Membranes, Plasma,Plasma Membranes
D002851 Chromatography, High Pressure Liquid Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed. Chromatography, High Performance Liquid,Chromatography, High Speed Liquid,Chromatography, Liquid, High Pressure,HPLC,High Performance Liquid Chromatography,High-Performance Liquid Chromatography,UPLC,Ultra Performance Liquid Chromatography,Chromatography, High-Performance Liquid,High-Performance Liquid Chromatographies,Liquid Chromatography, High-Performance
D003058 Cockroaches Insects of the order Dictyoptera comprising several families including Blaberidae, BLATTELLIDAE, Blattidae (containing the American cockroach PERIPLANETA americana), Cryptocercidae, and Polyphagidae. Blaberidae,Blattaria,Blattidae,Blattodea,Cryptocercidae,Dictyoptera,Polyphagidae,Cockroach,Blattarias,Blattodeas,Cockroache,Cockroachs,Dictyopteras

Related Publications

M J Little, and H Wilson, and C Zappia, and S Cestèle, and M I Tyler, and M F Martin-Eauclaire, and D Gordon, and G M Nicholson
delta-Atracotoxins from australian funnel-web spiders compete with scorpion alpha-toxin binding but differentially modulate alkaloid toxin activation of voltage-gated sodium channels.
October 1998, The Journal of biological chemistry,
M J Little, and H Wilson, and C Zappia, and S Cestèle, and M I Tyler, and M F Martin-Eauclaire, and D Gordon, and G M Nicholson
Variations in receptor site-3 on rat brain and insect sodium channels highlighted by binding of a funnel-web spider delta-atracotoxin.
March 2002, European journal of biochemistry,
M J Little, and H Wilson, and C Zappia, and S Cestèle, and M I Tyler, and M F Martin-Eauclaire, and D Gordon, and G M Nicholson
Tetrodotoxin reverses brevetoxin allosteric inhibition of scorpion alpha-toxin binding on rat brain sodium channels.
August 1996, The Journal of biological chemistry,
M J Little, and H Wilson, and C Zappia, and S Cestèle, and M I Tyler, and M F Martin-Eauclaire, and D Gordon, and G M Nicholson
Binding of an alpha scorpion toxin to insect sodium channels is not dependent on membrane potential.
January 1993, FEBS letters,
M J Little, and H Wilson, and C Zappia, and S Cestèle, and M I Tyler, and M F Martin-Eauclaire, and D Gordon, and G M Nicholson
Alpha-scorpion toxins binding on rat brain and insect sodium channels reveal divergent allosteric modulations by brevetoxin and veratridine.
June 1995, The Journal of biological chemistry,
M J Little, and H Wilson, and C Zappia, and S Cestèle, and M I Tyler, and M F Martin-Eauclaire, and D Gordon, and G M Nicholson
Effect of depolarization on binding kinetics of scorpion alpha-toxin highlights conformational changes of rat brain sodium channels.
December 2001, Biochemistry,
M J Little, and H Wilson, and C Zappia, and S Cestèle, and M I Tyler, and M F Martin-Eauclaire, and D Gordon, and G M Nicholson
The lethal toxin from Australian funnel-web spiders is encoded by an intronless gene.
January 2012, PloS one,
M J Little, and H Wilson, and C Zappia, and S Cestèle, and M I Tyler, and M F Martin-Eauclaire, and D Gordon, and G M Nicholson
The Deadly Toxin Arsenal of the Tree-Dwelling Australian Funnel-Web Spiders.
October 2022, International journal of molecular sciences,
M J Little, and H Wilson, and C Zappia, and S Cestèle, and M I Tyler, and M F Martin-Eauclaire, and D Gordon, and G M Nicholson
An 'Old World' scorpion beta-toxin that recognizes both insect and mammalian sodium channels.
June 2003, European journal of biochemistry,
M J Little, and H Wilson, and C Zappia, and S Cestèle, and M I Tyler, and M F Martin-Eauclaire, and D Gordon, and G M Nicholson
The binding of BmK IT2, a depressant insect-selective scorpion toxin on mammal and insect sodium channels.
November 2000, Neuroscience research,
Copied contents to your clipboard!