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To distinguish the bactericidal action of singlet oxygen (1O2) from hypohalous acids, wild-type and lycopene transformant E. coli strains were exposed to each of the oxidants and then bacterial viability was investigated. 1O2 was generated by chemical and enzymatic systems at pH 4.5. ExpoSure of wild-type E. coli to 1O2 caused a significant loss of E. coli viability due to inactivation of membrane respiratory chain enzymes by 1O2. This action of 1O2 could be attenuated by lycopene in the bacterial cell membrane. In the lycopene transformant strain of E. coli, inactivation of NADH oxidase and succinate oxidase by hypohalous acids were significantly suppressed, but E. coli viability was unaffected. Based on these findings, we suggest that phagocytic leukocytes produce 1O2 as a major bactericidal oxidant in the phagosome.
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N Misawa, and
K Fujimori, and
K Hori, and
Y Sano, and
Y Kambayashi, and
M Nakano
January 2006,
Antioxidants & redox signaling,
H Tatsuzawa, and
T Maruyama, and
N Misawa, and
K Fujimori, and
K Hori, and
Y Sano, and
Y Kambayashi, and
M Nakano
November 2020,
Environmental science & technology,
H Tatsuzawa, and
T Maruyama, and
N Misawa, and
K Fujimori, and
K Hori, and
Y Sano, and
Y Kambayashi, and
M Nakano
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H Tatsuzawa, and
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K Fujimori, and
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Y Kambayashi, and
M Nakano
May 2007,
Archives of biochemistry and biophysics,
H Tatsuzawa, and
T Maruyama, and
N Misawa, and
K Fujimori, and
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Y Sano, and
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M Nakano
December 1958,
Biochimica et biophysica acta,
H Tatsuzawa, and
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M Nakano
March 2016,
Journal of the American Chemical Society,
H Tatsuzawa, and
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N Misawa, and
K Fujimori, and
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M Nakano
November 2012,
Environmental science & technology,
H Tatsuzawa, and
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K Hori, and
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Y Kambayashi, and
M Nakano
June 1993,
Biochimica et biophysica acta,
H Tatsuzawa, and
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M Nakano
November 1986,
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H Tatsuzawa, and
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M Nakano
December 2013,
Free radical biology & medicine,
