BIOMAT Database Logo BIOMAT Database Logo

Prognostic significance of proliferative activity, DNA-ploidy, p53 and Ki-ras point mutations in colorectal liver metastases. 1998

A Russo, and M Migliavacca, and V Bazan, and N Maturi, and V Morello, and G Dardanoni, and G Modica, and P Bazan, and I Albanese, and M La Farina, and R M Tomasino
Department of Clinical Oncology Research, University of Palermo, Italy.

Paired colorectal liver metastases (CLM) and normal tissue samples from a consecutive series of 36 patients were studied prospectively. MIB-1 expression was studied by immunohistochemistry on paraffin-embedded sections. DNA ploidy and S-phase fraction (SPF) measurements were performed by flow cytometry on frozen tissues. Mutations within the p53 (exons 5-8) and c-Ki-ras (codons 12 and 13) genes were detected by PCR single-strand conformation polymorphism analysis followed by sequencing. A high correlation was observed between the MIB-1 LI and SPF value (rho=0.81; P<0.01). Moreover, p53 gene mutations were associated with either high MIB-1 LI and high SPF. In univariate analysis, SPF and MIB-1 levels were related to risk of death. The association between overall survival and DNA-ploidy or p53 mutations did not reach statistical significance, but a slightly better survival was observed for patients either with DNA-diploid tumours or without mutations (P=0.05 and P=0.06, respectively). SPF was shown by multivariate Cox model analysis to be an independent prognostic variable and thus it might be a useful prognostic factor in patients with CLM.

UI MeSH Term Description Entries
D007150 Immunohistochemistry Histochemical localization of immunoreactive substances using labeled antibodies as reagents. Immunocytochemistry,Immunogold Techniques,Immunogold-Silver Techniques,Immunohistocytochemistry,Immunolabeling Techniques,Immunogold Technics,Immunogold-Silver Technics,Immunolabeling Technics,Immunogold Silver Technics,Immunogold Silver Techniques,Immunogold Technic,Immunogold Technique,Immunogold-Silver Technic,Immunogold-Silver Technique,Immunolabeling Technic,Immunolabeling Technique,Technic, Immunogold,Technic, Immunogold-Silver,Technic, Immunolabeling,Technics, Immunogold,Technics, Immunogold-Silver,Technics, Immunolabeling,Technique, Immunogold,Technique, Immunogold-Silver,Technique, Immunolabeling,Techniques, Immunogold,Techniques, Immunogold-Silver,Techniques, Immunolabeling
D008113 Liver Neoplasms Tumors or cancer of the LIVER. Cancer of Liver,Hepatic Cancer,Liver Cancer,Cancer of the Liver,Cancer, Hepatocellular,Hepatic Neoplasms,Hepatocellular Cancer,Neoplasms, Hepatic,Neoplasms, Liver,Cancer, Hepatic,Cancer, Liver,Cancers, Hepatic,Cancers, Hepatocellular,Cancers, Liver,Hepatic Cancers,Hepatic Neoplasm,Hepatocellular Cancers,Liver Cancers,Liver Neoplasm,Neoplasm, Hepatic,Neoplasm, Liver
D008297 Male Males
D008875 Middle Aged An adult aged 45 - 64 years. Middle Age
D009687 Nuclear Proteins Proteins found in the nucleus of a cell. Do not confuse with NUCLEOPROTEINS which are proteins conjugated with nucleic acids, that are not necessarily present in the nucleus. Nucleolar Protein,Nucleolar Proteins,Nuclear Protein,Protein, Nuclear,Protein, Nucleolar,Proteins, Nuclear,Proteins, Nucleolar
D011003 Ploidies The degree of replication of the chromosome set in the karyotype. Ploidy
D011379 Prognosis A prediction of the probable outcome of a disease based on a individual's condition and the usual course of the disease as seen in similar situations. Prognostic Factor,Prognostic Factors,Factor, Prognostic,Factors, Prognostic,Prognoses
D011446 Prospective Studies Observation of a population for a sufficient number of persons over a sufficient number of years to generate incidence or mortality rates subsequent to the selection of the study group. Prospective Study,Studies, Prospective,Study, Prospective
D011905 Genes, ras Family of retrovirus-associated DNA sequences (ras) originally isolated from Harvey (H-ras, Ha-ras, rasH) and Kirsten (K-ras, Ki-ras, rasK) murine sarcoma viruses. Ras genes are widely conserved among animal species and sequences corresponding to both H-ras and K-ras genes have been detected in human, avian, murine, and non-vertebrate genomes. The closely related N-ras gene has been detected in human neuroblastoma and sarcoma cell lines. All genes of the family have a similar exon-intron structure and each encodes a p21 protein. Ha-ras Genes,Ki-ras Genes,N-ras Genes,c-Ha-ras Genes,c-Ki-ras Genes,c-N-ras Genes,ras Genes,v-Ha-ras Genes,v-Ki-ras Genes,H-ras Genes,H-ras Oncogenes,Ha-ras Oncogenes,K-ras Genes,K-ras Oncogenes,Ki-ras Oncogenes,N-ras Oncogenes,c-H-ras Genes,c-H-ras Proto-Oncogenes,c-Ha-ras Proto-Oncogenes,c-K-ras Genes,c-K-ras Proto-Oncogenes,c-Ki-ras Proto-Oncogenes,c-N-ras Proto-Oncogenes,ras Oncogene,v-H-ras Genes,v-H-ras Oncogenes,v-Ha-ras Oncogenes,v-K-ras Genes,v-K-ras Oncogenes,v-Ki-ras Oncogenes,Gene, Ha-ras,Gene, Ki-ras,Gene, v-Ha-ras,Gene, v-Ki-ras,Genes, Ha-ras,Genes, Ki-ras,Genes, N-ras,Genes, v-Ha-ras,Genes, v-Ki-ras,H ras Genes,H ras Oncogenes,H-ras Gene,H-ras Oncogene,Ha ras Genes,Ha ras Oncogenes,Ha-ras Gene,Ha-ras Oncogene,K ras Genes,K ras Oncogenes,K-ras Gene,K-ras Oncogene,Ki ras Genes,Ki ras Oncogenes,Ki-ras Gene,Ki-ras Oncogene,N ras Genes,N ras Oncogenes,N-ras Gene,N-ras Oncogene,c H ras Genes,c H ras Proto Oncogenes,c Ha ras Genes,c Ha ras Proto Oncogenes,c K ras Genes,c K ras Proto Oncogenes,c Ki ras Genes,c Ki ras Proto Oncogenes,c N ras Genes,c N ras Proto Oncogenes,c-H-ras Gene,c-H-ras Proto-Oncogene,c-Ha-ras Gene,c-Ha-ras Proto-Oncogene,c-K-ras Gene,c-K-ras Proto-Oncogene,c-Ki-ras Gene,c-Ki-ras Proto-Oncogene,c-N-ras Gene,c-N-ras Proto-Oncogene,ras Gene,ras Oncogenes,v H ras Genes,v H ras Oncogenes,v Ha ras Genes,v Ha ras Oncogenes,v K ras Genes,v K ras Oncogenes,v Ki ras Genes,v Ki ras Oncogenes,v-H-ras Gene,v-H-ras Oncogene,v-Ha-ras Gene,v-Ha-ras Oncogene,v-K-ras Gene,v-K-ras Oncogene,v-Ki-ras Gene,v-Ki-ras Oncogene
D005260 Female Females

Related Publications

A Russo, and M Migliavacca, and V Bazan, and N Maturi, and V Morello, and G Dardanoni, and G Modica, and P Bazan, and I Albanese, and M La Farina, and R M Tomasino
Mutations of Ki-ras and p53 genes in colorectal cancer and their prognostic significance.
November 1994, Gut,
A Russo, and M Migliavacca, and V Bazan, and N Maturi, and V Morello, and G Dardanoni, and G Modica, and P Bazan, and I Albanese, and M La Farina, and R M Tomasino
p53, Ki-ras, and DNA ploidy in human pancreatic ductal adenocarcinomas.
January 1996, Laboratory investigation; a journal of technical methods and pathology,
A Russo, and M Migliavacca, and V Bazan, and N Maturi, and V Morello, and G Dardanoni, and G Modica, and P Bazan, and I Albanese, and M La Farina, and R M Tomasino
Prognostic value of p53 overexpression and c-Ki-ras gene mutations in colorectal cancer.
January 1993, Gastroenterology,
A Russo, and M Migliavacca, and V Bazan, and N Maturi, and V Morello, and G Dardanoni, and G Modica, and P Bazan, and I Albanese, and M La Farina, and R M Tomasino
p53 mutations and DNA ploidy in colorectal adenocarcinomas.
January 1998, Analytical cellular pathology : the journal of the European Society for Analytical Cellular Pathology,
A Russo, and M Migliavacca, and V Bazan, and N Maturi, and V Morello, and G Dardanoni, and G Modica, and P Bazan, and I Albanese, and M La Farina, and R M Tomasino
[Molecular oncological study on DNA ploidy, Ki-ras point mutation, and p21 expression in colorectal cancer].
April 1994, Nihon Geka Gakkai zasshi,
A Russo, and M Migliavacca, and V Bazan, and N Maturi, and V Morello, and G Dardanoni, and G Modica, and P Bazan, and I Albanese, and M La Farina, and R M Tomasino
The prognostic significance of K-ras, p53, and APC mutations in colorectal carcinoma.
September 2005, Gut,
A Russo, and M Migliavacca, and V Bazan, and N Maturi, and V Morello, and G Dardanoni, and G Modica, and P Bazan, and I Albanese, and M La Farina, and R M Tomasino
Prospective study on prognostic significance of DNA ploidy and Ki-67 expression in colorectal cancer.
January 2007, Journal of biological regulators and homeostatic agents,
A Russo, and M Migliavacca, and V Bazan, and N Maturi, and V Morello, and G Dardanoni, and G Modica, and P Bazan, and I Albanese, and M La Farina, and R M Tomasino
Occurrence of Ki-ras and p53 mutations in primary colorectal tumors.
November 1991, Oncogene,
A Russo, and M Migliavacca, and V Bazan, and N Maturi, and V Morello, and G Dardanoni, and G Modica, and P Bazan, and I Albanese, and M La Farina, and R M Tomasino
Prognostic significance of p53 expression in relation to DNA ploidy in colorectal adenocarcinoma.
January 1993, Virchows Archiv. A, Pathological anatomy and histopathology,
A Russo, and M Migliavacca, and V Bazan, and N Maturi, and V Morello, and G Dardanoni, and G Modica, and P Bazan, and I Albanese, and M La Farina, and R M Tomasino
Prognostic significance of DNA ploidy and proliferative index in breast carcinoma.
June 1988, Archives of surgery (Chicago, Ill. : 1960),
Copied contents to your clipboard!