BIOMAT Database Logo BIOMAT Database Logo

Multiple truncated transcripts of TSG101 in gastrointestinal cancers. 1998

S Y Lin, and Y J Chen, and J G Chang
Graduate Institute of Medical Science, Taipei Medical College and Department of Internal Medicine, Taipei Medical College Hospital, Taiwan.

The candidate tumour suppressor gene TSG101, located on chromosome 11p15, has been associated with frequent intragenic deletion in uncultured primary human breast cancers. Using paired tumour and normal tissues from surgical specimens, we performed nested reverse transcriptase-polymerase chain reaction and direct sequencing to analyse TSG101 exons 1-6 from 32 gastric, 30 colorectal and 16 oesophageal cancers. Truncated transcripts, were found in both tumour and normal tissues from the stomach (15.6 and 12.5%), colon (13.3 and 3.3%) and oesophagus (25 and 25%). Multiple truncated transcripts in individual specimens were also observed. Two types of splicing patterns, one with three to six bases homology at the deletion junction (25.9%), the other with donor site 5' GT and acceptor site 3' AG (55.6%), were the most common patterns. We conclude that in gastrointestinal cancers, truncated transcripts of TSG101 gene occur not uncommonly and do so with a specific splicing pattern.

UI MeSH Term Description Entries
D009154 Mutation Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations. Mutations
D002880 Chromosomes, Human, Pair 11 A specific pair of GROUP C CHROMOSOMES of the human chromosome classification. Chromosome 11
D005091 Exons The parts of a transcript of a split GENE remaining after the INTRONS are removed. They are spliced together to become a MESSENGER RNA or other functional RNA. Mini-Exon,Exon,Mini Exon,Mini-Exons
D005770 Gastrointestinal Neoplasms Tumors or cancer of the GASTROINTESTINAL TRACT, from the MOUTH to the ANAL CANAL. Gastrointestinal Cancer,Cancer of Gastrointestinal Tract,Cancer of the Gastrointestinal Tract,Neoplasms, Gastrointestinal,Cancer, Gastrointestinal,Cancers, Gastrointestinal,Gastrointestinal Cancers,Gastrointestinal Neoplasm,Gastrointestinal Tract Cancer,Gastrointestinal Tract Cancers,Neoplasm, Gastrointestinal
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D014158 Transcription, Genetic The biosynthesis of RNA carried out on a template of DNA. The biosynthesis of DNA from an RNA template is called REVERSE TRANSCRIPTION. Genetic Transcription
D016147 Genes, Tumor Suppressor Genes that inhibit expression of the tumorigenic phenotype. They are normally involved in holding cellular growth in check. When tumor suppressor genes are inactivated or lost, a barrier to normal proliferation is removed and unregulated growth is possible. Antioncogenes,Cancer Suppressor Genes,Emerogenes,Genes, Cancer Suppressor,Genes, Growth Suppressor,Genes, Metastasis Suppressor,Growth Suppressor Genes,Metastasis Suppressor Genes,Tumor Suppressor Genes,Anti-Oncogenes,Genes, Onco-Suppressor,Oncogenes, Recessive,Tumor Suppressing Genes,Anti Oncogenes,Anti-Oncogene,Antioncogene,Cancer Suppressor Gene,Emerogene,Gene, Cancer Suppressor,Gene, Growth Suppressor,Gene, Metastasis Suppressor,Gene, Onco-Suppressor,Gene, Tumor Suppressing,Gene, Tumor Suppressor,Genes, Onco Suppressor,Genes, Tumor Suppressing,Growth Suppressor Gene,Metastasis Suppressor Gene,Onco-Suppressor Gene,Onco-Suppressor Genes,Oncogene, Recessive,Recessive Oncogene,Recessive Oncogenes,Suppressor Gene, Cancer,Suppressor Gene, Growth,Suppressor Gene, Metastasis,Suppressor Genes, Cancer,Suppressor Genes, Growth,Suppressor Genes, Metastasis,Tumor Suppressing Gene,Tumor Suppressor Gene
D017385 Sequence Homology The degree of similarity between sequences. Studies of AMINO ACID SEQUENCE HOMOLOGY and NUCLEIC ACID SEQUENCE HOMOLOGY provide useful information about the genetic relatedness of genes, gene products, and species. Homologous Sequences,Homologs, Sequence,Sequence Homologs,Homolog, Sequence,Homologies, Sequence,Homologous Sequence,Homology, Sequence,Sequence Homolog,Sequence Homologies,Sequence, Homologous,Sequences, Homologous
D017422 Sequence Analysis, DNA A multistage process that includes cloning, physical mapping, subcloning, determination of the DNA SEQUENCE, and information analysis. DNA Sequence Analysis,Sequence Determination, DNA,Analysis, DNA Sequence,DNA Sequence Determination,DNA Sequence Determinations,DNA Sequencing,Determination, DNA Sequence,Determinations, DNA Sequence,Sequence Determinations, DNA,Analyses, DNA Sequence,DNA Sequence Analyses,Sequence Analyses, DNA,Sequencing, DNA
D020133 Reverse Transcriptase Polymerase Chain Reaction A variation of the PCR technique in which cDNA is made from RNA via reverse transcription. The resultant cDNA is then amplified using standard PCR protocols. Polymerase Chain Reaction, Reverse Transcriptase,Reverse Transcriptase PCR,PCR, Reverse Transcriptase,Transcriptase PCR, Reverse

Related Publications

S Y Lin, and Y J Chen, and J G Chang
Truncated TSG101 transcripts in human leukemia and lymphoma cell lines.
February 2000, Journal of cancer research and clinical oncology,
S Y Lin, and Y J Chen, and J G Chang
Analysis of TSG101 tumour susceptibility gene transcripts in cervical and endometrial cancers.
February 1999, British journal of cancer,
S Y Lin, and Y J Chen, and J G Chang
Truncated TSG101 transcripts are present in peripheral blood from both familial breast cancer patients and controls.
June 1998, European journal of cancer (Oxford, England : 1990),
S Y Lin, and Y J Chen, and J G Chang
Expression of truncated FHIT transcripts in cervical cancers and in normal human cells.
August 2001, Oncogene,
S Y Lin, and Y J Chen, and J G Chang
Frequent abnormalities of TSG101 transcripts in human prostate cancer.
December 1997, Oncogene,
S Y Lin, and Y J Chen, and J G Chang
Evidence that TSG101 aberrant transcripts are PCR artifacts.
July 1998, Biochemical and biophysical research communications,
S Y Lin, and Y J Chen, and J G Chang
Absence of TSG101 transcript abnormalities in human cancers.
May 1998, Oncogene,
S Y Lin, and Y J Chen, and J G Chang
Truncated FGFR2 is a clinically actionable oncogene in multiple cancers.
August 2022, Nature,
S Y Lin, and Y J Chen, and J G Chang
Are aberrant transcripts of FHIT, TSG101, and PTEN/MMAC1 oncogenesis related?
May 1999, International journal of molecular medicine,
S Y Lin, and Y J Chen, and J G Chang
Publisher Correction: Truncated FGFR2 is a clinically actionable oncogene in multiple cancers.
September 2022, Nature,
Copied contents to your clipboard!