Biomaterial Database

Simultaneous determination of ascorbic acid, pyridoxine hydrochloride, and tyrosine by derivative UV spectrophotometry. 1998

M Surmeian

Institute of Chemical and Pharmaceutical Research, Bucharest-3, Romania.

A new UV spectrophotometric method for resolving a three-component drug mixture of ascorbic acid (I), pyridoxine hydrochloride (II), and tyrosine (III), on the basis of the first-derivative UV spectra and zero-crossing technique, is described. Beer's law was obeyed in the concentration range of 7.0-15.0, 0.25-1.0, and 8.0-40.0 micrograms/ml for I, II, and III, respectively. Lower limits of detection at the 95% confidence level were 2.88, 0.69, and 5.43 micrograms/ml, respectively. The advantages of the proposed method (speed, easy sample preparation, and lower cost per analysis) also include its application to the content uniformity and dissolution test of a three-component mixture of drugs.

UI	MeSH Term	Description	Entries
D011736	Pyridoxine	The 4-methanol form of VITAMIN B 6 which is converted to PYRIDOXAL PHOSPHATE which is a coenzyme for synthesis of amino acids, neurotransmitters (serotonin, norepinephrine), sphingolipids, aminolevulinic acid. Although pyridoxine and Vitamin B 6 are still frequently used as synonyms, especially by medical researchers, this practice is erroneous and sometimes misleading (EE Snell; Ann NY Acad Sci, vol 585 pg 1, 1990).	Pyridoxin,Pyridoxine Hydrochloride,Pyridoxol,Pyridoxol Hydrochloride,Rodex
D002138	Calibration	Determination, by measurement or comparison with a standard, of the correct value of each scale reading on a meter or other measuring instrument; or determination of the settings of a control device that correspond to particular values of voltage, current, frequency or other output.	Calibrations
D001205	Ascorbic Acid	A six carbon compound related to glucose. It is found naturally in citrus fruits and many vegetables. Ascorbic acid is an essential nutrient in human diets, and necessary to maintain connective tissue and bone. Its biologically active form, vitamin C, functions as a reducing agent and coenzyme in several metabolic pathways. Vitamin C is considered an antioxidant.	Vitamin C,Ascorbic Acid, Monosodium Salt,Ferrous Ascorbate,Hybrin,L-Ascorbic Acid,Magnesium Ascorbate,Magnesium Ascorbicum,Magnesium di-L-Ascorbate,Magnorbin,Sodium Ascorbate,Acid, Ascorbic,Acid, L-Ascorbic,Ascorbate, Ferrous,Ascorbate, Magnesium,Ascorbate, Sodium,L Ascorbic Acid,Magnesium di L Ascorbate,di-L-Ascorbate, Magnesium
D012995	Solubility	The ability of a substance to be dissolved, i.e. to form a solution with another substance. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)	Solubilities
D013056	Spectrophotometry, Ultraviolet	Determination of the spectra of ultraviolet absorption by specific molecules in gases or liquids, for example Cl2, SO2, NO2, CS2, ozone, mercury vapor, and various unsaturated compounds. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)	Ultraviolet Spectrophotometry
D013608	Tablets, Enteric-Coated	Tablets coated with material that delays release of the medication until after they leave the stomach. (Dorland, 28th ed)	Enteric-Coated Tablets,Tablets, Enteric Coated
D014443	Tyrosine	A non-essential amino acid. In animals it is synthesized from PHENYLALANINE. It is also the precursor of EPINEPHRINE; THYROID HORMONES; and melanin.	L-Tyrosine,Tyrosine, L-isomer,para-Tyrosine,L Tyrosine,Tyrosine, L isomer,para Tyrosine