In this article, the methodologies and clinical applications of microperimetry performed by scanning laser ophthalmoscope (SLO) are described. This technique provides functional results by direct visualization of the macular area. We present the most interesting data about clinical applications in ocular pathology. SLO microperimetry is a new diagnostic tool in ophthalmic practice. It permits an exact, point-to-point correspondence between fundus image and perimetric results, impossible to achieve by projection perimetry, so representing the most suitable device for simultaneous fundus imaging and psychophysical testing. Microperimetry is performed by SLO (Rodenstock),1 which permits other applications. SLO microperimetry allows the real-time functional study of retinal sensitivity by direct ophthalmoscopic control of the retinal surface. Foveal or excentric fixation can be assessed as well. Instability of fixation during computerized perimetry is a possible misleading factor resulting in unexplainable findings, especially in eyes with decreased visual acuity. The main characteristic of microperimetry is the ability to see the stimuli presented on the retina in real time: this permits an accurate monitoring of fixation and correlation of anatomical or pathological features directly with retinal function.