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A flow cytometric method to detect and study human eosinophils in whole blood was established. Normal subjects and patients with various types of eosinophilia (hypereosinophilic syndromes, allergic diseases, dermatitis, Hodgkin's Disease, parasitosis) were studied. Whole blood samples were treated for 10 minutes at room temperature with a commercially available reagent (FACS Lysing Solution, Becton Dickinson) which acts both as a fixative and as a lysing agent. Eosinophils were identified as a granulocytic subpopulation with higher SSC and FSC properties. This cell population was characterized by evident autofluorescence and hypodiploid DNA features after propidium iodide staining. The purity of the eosinophil population sorted after electronic gating was close to 100%. A very significant correlation between eosinophil counting by our whole blood method and other two assays, namely routine automatic counting by the H*3 Bayer System and eosinophil detection by depolarized SSC, was obtained. The phagocytic properties of eosinophils were also studied by means of a commercially available diagnostic kit, thus demonstrating that our method is also suitable for the study of those granulocytic functions which can be evaluated by flow cytometry.
G Carulli, and
S Sbrana, and
A Azzarà, and
S Minnucci, and
C Angiolini, and
A Marini, and
F Ambrogi
January 2014,
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F Ambrogi
October 1999,
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F Ambrogi
May 2002,
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January 1985,
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Cytometry. Part A : the journal of the International Society for Analytical Cytology,
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F Ambrogi
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F Ambrogi
March 1998,
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G Carulli, and
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F Ambrogi
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Experimental and toxicologic pathology : official journal of the Gesellschaft fur Toxikologische Pathologie,
G Carulli, and
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F Ambrogi
March 1992,
The Journal of antimicrobial chemotherapy,
