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In the rat adrenal gland, we previously observed that SNAP-25 is not restricted to the plasmalemma in noradrenergic cells as it is in adrenergic cells, and hypothesized that SNAP-25 isoform expression is different in the two phenotypes. Expression of SNAP-25 isoforms and SNAP-23 was examined by immunoblotting, immunofluorescence, and RT-PCR. Amplifications of SNAP-25 mRNAs were combined with Southern hybridization, restriction enzyme analysis, and sequencing of cloned PCR products to compare SNAP-25 isoform expression in rat and bovine adrenal glands. SNAP-25 and SNAP-23 mRNA and protein are expressed in the glands; SNAP-23 is enriched in the adrenal cortex, whereas SNAP-25 is restricted to the adrenal medulla. Furthermore, high levels of SNAP-25 and low levels of SNAP-23 are observed in the PC12 cells, whereas both SNAP-25 and SNAP-23 are expressed in adrenal medullary cultures. In all extracts, the SNAP-23 mRNA corresponded to SNAP-23a. SNAP-25a is the major form expressed in rat adrenal glands (75%), as it is in PC12 cells (80%), but both SNAP-25a and SNAP-25b (40% vs. 60%) are expressed in bovine adrenal medulla in situ and in culture. In addition, an enriched population of adrenergic cells (93%) expressed a higher level of SNAP-25b (70%), suggesting that this isoform may not be restricted to fast neurotransmission.
N J Grant, and
R Hepp, and
W Krause, and
D Aunis, and
P Oehme, and
K Langley
April 1996,
The Journal of comparative neurology,
N J Grant, and
R Hepp, and
W Krause, and
D Aunis, and
P Oehme, and
K Langley
July 2003,
Cell,
N J Grant, and
R Hepp, and
W Krause, and
D Aunis, and
P Oehme, and
K Langley
May 1999,
Biochemical and biophysical research communications,
N J Grant, and
R Hepp, and
W Krause, and
D Aunis, and
P Oehme, and
K Langley
February 1999,
Journal of neurochemistry,
N J Grant, and
R Hepp, and
W Krause, and
D Aunis, and
P Oehme, and
K Langley
February 1995,
Proceedings of the National Academy of Sciences of the United States of America,
N J Grant, and
R Hepp, and
W Krause, and
D Aunis, and
P Oehme, and
K Langley
August 2001,
Biochemistry,
N J Grant, and
R Hepp, and
W Krause, and
D Aunis, and
P Oehme, and
K Langley
February 1998,
The Journal of biological chemistry,
N J Grant, and
R Hepp, and
W Krause, and
D Aunis, and
P Oehme, and
K Langley
June 2008,
Molecular and cellular endocrinology,
N J Grant, and
R Hepp, and
W Krause, and
D Aunis, and
P Oehme, and
K Langley
February 2015,
Neuroscience,
N J Grant, and
R Hepp, and
W Krause, and
D Aunis, and
P Oehme, and
K Langley
August 1998,
General and comparative endocrinology,
