-transparent.png){kind=logo}
Cholesterol can be determined colorimetrically in dairy products at levels of larger than or equal to 10 mug (coefficient of variation = 5.3%) with an o-phthalaldehyde reagent when non-cholesterol lipids are eliminated prior to color development. Absorbance for 2 mg tripalmitin was found to be equivalent to about 20 mug cholesterol. Saponification followed by hexane extraction removed interfering lipids. Using the described procedure, 238 individual raw milk samples were found to contain 144.4+/-37.9 mug cholesterol/ml, while their skim milk portions had 26.5+/-6.4 mug cholesterol/ml (mean +/- standard deviation). The o-phthalaldehyde cholesterol estimates agreed with those obtained by a gas-liquid chromatographic procedure when cheese and ice cream were analyzed by the colorimetric procedure with and without prior fat extraction.
K C Bachman, and
J H Lin, and
C J Wilcox
June 1953,
American journal of clinical pathology,
K C Bachman, and
J H Lin, and
C J Wilcox
March 1978,
Gigiena i sanitariia,
K C Bachman, and
J H Lin, and
C J Wilcox
June 1973,
Journal of pharmaceutical sciences,
K C Bachman, and
J H Lin, and
C J Wilcox
September 1972,
Journal - Association of Official Analytical Chemists,
K C Bachman, and
J H Lin, and
C J Wilcox
October 1979,
Journal of food protection,
K C Bachman, and
J H Lin, and
C J Wilcox
November 2022,
Food chemistry,
K C Bachman, and
J H Lin, and
C J Wilcox
April 1969,
Journal of dairy science,
K C Bachman, and
J H Lin, and
C J Wilcox
March 1973,
Journal of the American Dietetic Association,
K C Bachman, and
J H Lin, and
C J Wilcox
April 1978,
Food and cosmetics toxicology,
K C Bachman, and
J H Lin, and
C J Wilcox
August 2010,
Food & nutrition research,
