The effect of flow on fluid filtration across an arterial wall was investigated in the canine common carotid artery. The arteries were cannulated in situ to maintain their in vivo length and endothelium intact. The excised vessels were pressurized at 120 mmHg through an overflow head-tank system that provided a constant flow rate to the perfused vessels. Filtration rates across the walls of the carotid arteries tested were measured under 4 different experimental conditions: 1) albumin-free Krebs solution under absent flow condition; 2) albumin-free Krebs solution with flow (148 +/- 8 ml/min); 3) Krebs solution containing 1.0 g/dl bovine serum albumin under absent flow condition; 4) Krebs solution containing 1.0 g/dl bovine serum albumin with flow (148 +/- 8 ml/min). Under absent flow conditions, the addition of albumin to the Krebs solution (1.0 g/dl) led to an approximate 25% drop in filtration rate (p < 0.001). It was found that fluid flow affected the filtration rate of the albumin solution, yet failed to affect the filtration rate of albumin-free solution across the arterial wall. The present study suggests that the change in filtration rate of the albumin solution under flow condition may indicate the change in the luminal surface concentration of albumin due to flow.