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Biphasic translocation of a 70 kDa heat shock protein in human cytomegalovirus-infected cells. 1999

E Ohgitani, and K Kobayashi, and K Takeshita, and J Imanishi

Human cytomegalovirus (HCMV) reportedly induces the expression of a 70 kDa heat shock protein (hsp70) with no known function in the virus replication cycle. We report here a remarkably specific translocation pattern of hsp70 during HCMV infection of human diploid fibroblasts. Immunofluorescent observation and Western blotting of subcellular fractions revealed nuclear localization of hsp70 early in infection and predominantly cytoplasmic localization of hsp70 late in infection. Treatment of HCMV-infected cells with cycloheximide followed by treatment with actinomycin D allowed virus immediate-early gene expression but inhibited hsp70 nuclear localization. Phosphonoacetic acid and tunicamycin, both of which reportedly inhibit HCMV DNA replication, did not inhibit HCMV-induced nuclear localization of hsp70 but inhibited hsp70 translocation from the nucleus to the cytoplasm. These results indicate a correlation between HCMV multiplication and hsp70 localization, suggesting that hsp70 may play a role in HCMV multiplication.

UI MeSH Term Description Entries
D002460 Cell Line Established cell cultures that have the potential to propagate indefinitely. Cell Lines,Line, Cell,Lines, Cell
D003587 Cytomegalovirus A genus of the family HERPESVIRIDAE, subfamily BETAHERPESVIRINAE, infecting the salivary glands, liver, spleen, lungs, eyes, and other organs, in which they produce characteristically enlarged cells with intranuclear inclusions. Infection with Cytomegalovirus is also seen as an opportunistic infection in AIDS. Herpesvirus 5, Human,Human Herpesvirus 5,Salivary Gland Viruses,HHV 5,Herpesvirus 5 (beta), Human,Cytomegaloviruses,Salivary Gland Virus,Virus, Salivary Gland,Viruses, Salivary Gland
D006360 Heat-Shock Proteins Proteins which are synthesized in eukaryotic organisms and bacteria in response to hyperthermia and other environmental stresses. They increase thermal tolerance and perform functions essential to cell survival under these conditions. Stress Protein,Stress Proteins,Heat-Shock Protein,Heat Shock Protein,Heat Shock Proteins,Protein, Stress
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D001692 Biological Transport The movement of materials (including biochemical substances and drugs) through a biological system at the cellular level. The transport can be across cell membranes and epithelial layers. It also can occur within intracellular compartments and extracellular compartments. Transport, Biological,Biologic Transport,Transport, Biologic
D014764 Viral Proteins Proteins found in any species of virus. Gene Products, Viral,Viral Gene Products,Viral Gene Proteins,Viral Protein,Protein, Viral,Proteins, Viral
D050883 HSC70 Heat-Shock Proteins A constitutively expressed subfamily of the HSP70 heat-shock proteins. They preferentially bind and release hydrophobic peptides by an ATP-dependent process and are involved in post-translational PROTEIN TRANSLOCATION. Heat-Shock Cognate 70 Protein,Hsc70 Protein,70 kDa Heat-Shock Cognate Protein,Clathrin Uncoating Protein,HSC73 Protein,70 kDa Heat Shock Cognate Protein,HSC70 Heat Shock Proteins,Heat Shock Cognate 70 Protein,Heat-Shock Proteins, HSC70,Uncoating Protein, Clathrin
D017874 Immediate-Early Proteins Proteins that are coded by immediate-early genes, in the absence of de novo protein synthesis. The term was originally used exclusively for viral regulatory proteins that were synthesized just after viral integration into the host cell. It is also used to describe cellular proteins which are synthesized immediately after the resting cell is stimulated by extracellular signals. Proteins, Immediate-Early,Immediate Early Proteins,Proteins, Immediate Early
D018840 HSP70 Heat-Shock Proteins A class of MOLECULAR CHAPERONES found in both prokaryotes and in several compartments of eukaryotic cells. These proteins can interact with polypeptides during a variety of assembly processes in such a way as to prevent the formation of nonfunctional structures. Heat-Shock Proteins 70,Heat Shock 70 kDa Protein,Heat-Shock Protein 70,HSP70 Heat Shock Proteins,Heat Shock Protein 70,Heat Shock Proteins 70,Heat-Shock Proteins, HSP70

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