BIOMAT Database Logo BIOMAT Database Logo

111Indium-labeled monoclonal antibody K1: biodistribution study in nude mice bearing a human carcinoma xenograft expressing mesothelin. 1999

R Hassan, and C Wu, and M W Brechbiel, and I Margulies, and R J Kreitman, and I Pastan
Lombardi Cancer Center, Georgetown University Medical Center, Washington, DC, USA.

The monoclonal antibody (MAb) K1 is a murine IgG1 that recognizes mesothelin, a differentiation antigen present on mesothelium which is highly expressed on cancers derived from mesothelium, including most ovarian cancers and epithelioid mesotheliomas. MAb K1 was conjugated to 2-(p-isothiocyanatobenzyl)-cyclohexyl- diethylenetriaminepentaacetic acid and labeled with 111In. The biodistribution of 111In-K1 was studied in athymic nude mice bearing 2 s.c. tumors, one expressing a stably transfected plasmid encoding mesothelin and one composed of the parental untransfected A431 epidermoid carcinoma cells which do not express mesothelin. Tumor-bearing mice were given an i.v. injection of 111In-K1 and killed at different time points to determine the uptake of radiolabeled antibody. Significantly higher uptake was seen in antigen-positive tumors at all time points, with peak values at 72 hr (52.9% vs. 8% of the injected dose/g tissue for antigen-positive and antigen-negative tumors, respectively). Uptake in antigen-positive tumors was higher than the blood level at all time points, and the tumors contained a high level of the radiolabeled MAb even at 7 days (28.6% of the injected dose/g tumor).

UI MeSH Term Description Entries
D007074 Immunoglobulin G The major immunoglobulin isotype class in normal human serum. There are several isotype subclasses of IgG, for example, IgG1, IgG2A, and IgG2B. Gamma Globulin, 7S,IgG,IgG Antibody,Allerglobuline,IgG(T),IgG1,IgG2,IgG2A,IgG2B,IgG3,IgG4,Immunoglobulin GT,Polyglobin,7S Gamma Globulin,Antibody, IgG,GT, Immunoglobulin
D007205 Indium Radioisotopes Unstable isotopes of indium that decay or disintegrate emitting radiation. In atoms with atomic weights 106-112, 113m, 114, and 116-124 are radioactive indium isotopes. Radioisotopes, Indium
D008562 Membrane Glycoproteins Glycoproteins found on the membrane or surface of cells. Cell Surface Glycoproteins,Surface Glycoproteins,Cell Surface Glycoprotein,Membrane Glycoprotein,Surface Glycoprotein,Glycoprotein, Cell Surface,Glycoprotein, Membrane,Glycoprotein, Surface,Glycoproteins, Cell Surface,Glycoproteins, Membrane,Glycoproteins, Surface,Surface Glycoprotein, Cell,Surface Glycoproteins, Cell
D008819 Mice, Nude Mutant mice homozygous for the recessive gene "nude" which fail to develop a thymus. They are useful in tumor studies and studies on immune responses. Athymic Mice,Mice, Athymic,Nude Mice,Mouse, Athymic,Mouse, Nude,Athymic Mouse,Nude Mouse
D010051 Ovarian Neoplasms Tumors or cancer of the OVARY. These neoplasms can be benign or malignant. They are classified according to the tissue of origin, such as the surface EPITHELIUM, the stromal endocrine cells, and the totipotent GERM CELLS. Cancer of Ovary,Ovarian Cancer,Cancer of the Ovary,Neoplasms, Ovarian,Ovary Cancer,Ovary Neoplasms,Cancer, Ovarian,Cancer, Ovary,Cancers, Ovarian,Cancers, Ovary,Neoplasm, Ovarian,Neoplasm, Ovary,Neoplasms, Ovary,Ovarian Cancers,Ovarian Neoplasm,Ovary Cancers,Ovary Neoplasm
D002294 Carcinoma, Squamous Cell A carcinoma derived from stratified SQUAMOUS EPITHELIAL CELLS. It may also occur in sites where glandular or columnar epithelium is normally present. (From Stedman, 25th ed) Carcinoma, Epidermoid,Carcinoma, Planocellular,Carcinoma, Squamous,Squamous Cell Carcinoma,Carcinomas, Epidermoid,Carcinomas, Planocellular,Carcinomas, Squamous,Carcinomas, Squamous Cell,Epidermoid Carcinoma,Epidermoid Carcinomas,Planocellular Carcinoma,Planocellular Carcinomas,Squamous Carcinoma,Squamous Carcinomas,Squamous Cell Carcinomas
D005260 Female Females
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000090204 Mesothelin An ANTIGEN present on the surface of certain types of normal cells and overexpressed in several human tumors, including OVARIAN CANCER. CAK-1 Antigen,Megakaryocyte Potentiating Factor,Antigen, CAK-1,CAK 1 Antigen,Factor, Megakaryocyte Potentiating,Potentiating Factor, Megakaryocyte
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia

Related Publications

R Hassan, and C Wu, and M W Brechbiel, and I Margulies, and R J Kreitman, and I Pastan
Preclinical evaluation of 111In-labeled B3 monoclonal antibody: biodistribution and imaging studies in nude mice bearing human epidermoid carcinoma xenografts.
June 1993, Cancer research,
R Hassan, and C Wu, and M W Brechbiel, and I Margulies, and R J Kreitman, and I Pastan
[Biodistribution of 125I labeled monoclonal antibody against gamma seminoprotein in the nude mice bearing human benign prostatic hyperplasia xenografts].
July 1993, Kaku igaku. The Japanese journal of nuclear medicine,
R Hassan, and C Wu, and M W Brechbiel, and I Margulies, and R J Kreitman, and I Pastan
High-specific-activity 111In-labeled anticarcinoembryonic antigen monoclonal antibody: biodistribution and imaging in nude mice bearing human colon cancer xenografts.
November 1985, Cancer research,
R Hassan, and C Wu, and M W Brechbiel, and I Margulies, and R J Kreitman, and I Pastan
[Biodistribution and radioimmunoimaging of monoclonal antibody to human lung adenocarcinoma in tumor-bearing nude mice].
May 1990, Zhonghua zhong liu za zhi [Chinese journal of oncology],
R Hassan, and C Wu, and M W Brechbiel, and I Margulies, and R J Kreitman, and I Pastan
Biodistribution of a benzoporphyrin derivative-monoclonal antibody conjugate in A549-tumor-bearing nude mice.
January 1993, Biotechnology therapeutics,
R Hassan, and C Wu, and M W Brechbiel, and I Margulies, and R J Kreitman, and I Pastan
Biodistribution of monoclonal antibody A7 and its F(ab')2 fragment in athymic nude mice bearing human pancreatic carcinoma.
July 1992, Journal of surgical oncology,
R Hassan, and C Wu, and M W Brechbiel, and I Margulies, and R J Kreitman, and I Pastan
[Biodistribution, pharmacokinetics and imaging of 131I-labeled HMD4 human McAb in human ovarian carcinoma xenograft in nude mice].
March 1990, Zhonghua fu chan ke za zhi,
R Hassan, and C Wu, and M W Brechbiel, and I Margulies, and R J Kreitman, and I Pastan
Dynamics of monoclonal antibody distribution and prolonged tumour localisation in nude mice bearing a human CEA-producing colon carcinoma xenograft.
January 1986, Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine,
R Hassan, and C Wu, and M W Brechbiel, and I Margulies, and R J Kreitman, and I Pastan
Biodistribution of an antibody-enzyme conjugate for antibody-directed enzyme prodrug therapy in nude mice bearing a human colon adenocarcinoma xenograft.
January 1997, Cancer chemotherapy and pharmacology,
R Hassan, and C Wu, and M W Brechbiel, and I Margulies, and R J Kreitman, and I Pastan
[Immunoscintigraphy of anti-type IV collagenase monoclonal antibody in nude mice bearing human lung cancer xenograft].
December 2003, Ai zheng = Aizheng = Chinese journal of cancer,
Copied contents to your clipboard!