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A mutant angiotensinogen, L11V, in which Val11 was substituted for Leu11 of ovine angiotensinogen was prepared to have the same scissile peptide bond as the human one. The mutation didn't vary Km and kcat of human renin for the ovine substrate, but decreased those of rat renin to one half and one fortieth, respectively. Distances between the P1' subsite of angiotensinogens and the 224th (human renin numbering) residue in the S1' subsite of renins were estimated by molecular modelings. The marked decrease in kcat of rat renin for L11V could be attributed to the elongated distance between Val11 of L11V and Val221 of rat renin. It was also suggested that the distance is the reason why the human substrate cannot be cleaved by heterologous renins.
Y Inui, and
T Orihashi, and
E Okada, and
T Nakagawa, and
A Ebihara, and
F Suzuki, and
Y Nakamura
November 1969,
The American journal of physiology,
Y Inui, and
T Orihashi, and
E Okada, and
T Nakagawa, and
A Ebihara, and
F Suzuki, and
Y Nakamura
November 1969,
Endocrinology,
Y Inui, and
T Orihashi, and
E Okada, and
T Nakagawa, and
A Ebihara, and
F Suzuki, and
Y Nakamura
November 1985,
The Journal of clinical investigation,
Y Inui, and
T Orihashi, and
E Okada, and
T Nakagawa, and
A Ebihara, and
F Suzuki, and
Y Nakamura
July 1988,
Journal of medicinal chemistry,
Y Inui, and
T Orihashi, and
E Okada, and
T Nakagawa, and
A Ebihara, and
F Suzuki, and
Y Nakamura
April 2019,
Heliyon,
Y Inui, and
T Orihashi, and
E Okada, and
T Nakagawa, and
A Ebihara, and
F Suzuki, and
Y Nakamura
March 1995,
Nihon rinsho. Japanese journal of clinical medicine,
Y Inui, and
T Orihashi, and
E Okada, and
T Nakagawa, and
A Ebihara, and
F Suzuki, and
Y Nakamura
March 1975,
The American journal of physiology,
Y Inui, and
T Orihashi, and
E Okada, and
T Nakagawa, and
A Ebihara, and
F Suzuki, and
Y Nakamura
July 1984,
Biochemical and biophysical research communications,
Y Inui, and
T Orihashi, and
E Okada, and
T Nakagawa, and
A Ebihara, and
F Suzuki, and
Y Nakamura
April 1997,
Hypertension (Dallas, Tex. : 1979),
Y Inui, and
T Orihashi, and
E Okada, and
T Nakagawa, and
A Ebihara, and
F Suzuki, and
Y Nakamura
January 1971,
Acta physiologica latino americana,
