BIOMAT Database Logo BIOMAT Database Logo

A novel glycerol kinase from Flavobacterium meningosepticum: characterization, gene cloning and primary structure. 1998

S Sakasegawa, and I Yoshioka, and S Koga, and M Takahashi, and K Matsumoto, and H Misaki, and T Ohshima
Asahi Chemical Industry Co. Ltd., Shizuoka, Japan. a9310932@ut.asahi-kasei.co.jp

A thermostable glycerol kinase (FGK) was purified 34-fold to homogeneity from Flavobacterium meningosepticum. The molecular masses of the enzyme were 200 kDa by gel filtration and 50 kDa by SDS-PAGE. The Km for glycerol and ATP were 0.088 and 0.030 mM, respectively. The enzyme was stable at 65 degrees C for 10 min and at 37 degrees C for two weeks. The enzyme gene was cloned into Escherichia coli and its complete DNA was sequenced. The FGK gene consists of an open reading frame of 1494-bp encoding a protein of 498 amino acids. The deduced amino acid sequence of the gene had 40-60% similarity to those of glycerol kinases from other origins and the amino acid sequence of the putative active site residue reported for E. coli GK is identical to the corresponding sequence of FGK except for one amino acid residue.

UI MeSH Term Description Entries
D007526 Isoelectric Point The pH in solutions of proteins and related compounds at which the dipolar ions are at a maximum. Isoelectric Points,Point, Isoelectric,Points, Isoelectric
D007700 Kinetics The rate dynamics in chemical or physical systems.
D008969 Molecular Sequence Data Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories. Sequence Data, Molecular,Molecular Sequencing Data,Data, Molecular Sequence,Data, Molecular Sequencing,Sequencing Data, Molecular
D008970 Molecular Weight The sum of the weight of all the atoms in a molecule. Molecular Weights,Weight, Molecular,Weights, Molecular
D002847 Chromatography, Agarose A method of gel filtration chromatography using agarose, the non-ionic component of agar, for the separation of compounds with molecular weights up to several million. Chromatography, Sepharose,Agarose Chromatography,Sepharose Chromatography,Agarose Chromatographies,Chromatographies, Agarose,Chromatographies, Sepharose,Sepharose Chromatographies
D002850 Chromatography, Gel Chromatography on non-ionic gels without regard to the mechanism of solute discrimination. Chromatography, Exclusion,Chromatography, Gel Permeation,Chromatography, Molecular Sieve,Gel Filtration,Gel Filtration Chromatography,Chromatography, Size Exclusion,Exclusion Chromatography,Gel Chromatography,Gel Permeation Chromatography,Molecular Sieve Chromatography,Chromatography, Gel Filtration,Exclusion Chromatography, Size,Filtration Chromatography, Gel,Filtration, Gel,Sieve Chromatography, Molecular,Size Exclusion Chromatography
D002851 Chromatography, High Pressure Liquid Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed. Chromatography, High Performance Liquid,Chromatography, High Speed Liquid,Chromatography, Liquid, High Pressure,HPLC,High Performance Liquid Chromatography,High-Performance Liquid Chromatography,UPLC,Ultra Performance Liquid Chromatography,Chromatography, High-Performance Liquid,High-Performance Liquid Chromatographies,Liquid Chromatography, High-Performance
D003001 Cloning, Molecular The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells. Molecular Cloning
D004591 Electrophoresis, Polyacrylamide Gel Electrophoresis in which a polyacrylamide gel is used as the diffusion medium. Polyacrylamide Gel Electrophoresis,SDS-PAGE,Sodium Dodecyl Sulfate-PAGE,Gel Electrophoresis, Polyacrylamide,SDS PAGE,Sodium Dodecyl Sulfate PAGE,Sodium Dodecyl Sulfate-PAGEs
D004926 Escherichia coli A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc. Alkalescens-Dispar Group,Bacillus coli,Bacterium coli,Bacterium coli commune,Diffusely Adherent Escherichia coli,E coli,EAggEC,Enteroaggregative Escherichia coli,Enterococcus coli,Diffusely Adherent E. coli,Enteroaggregative E. coli,Enteroinvasive E. coli,Enteroinvasive Escherichia coli

Related Publications

S Sakasegawa, and I Yoshioka, and S Koga, and M Takahashi, and K Matsumoto, and H Misaki, and T Ohshima
Prolyl endopeptidase from Flavobacterium meningosepticum: cloning and sequencing of the enzyme gene.
December 1991, Journal of biochemistry,
S Sakasegawa, and I Yoshioka, and S Koga, and M Takahashi, and K Matsumoto, and H Misaki, and T Ohshima
Crystal structure of glycosylasparaginase from Flavobacterium meningosepticum.
March 1998, Protein science : a publication of the Protein Society,
S Sakasegawa, and I Yoshioka, and S Koga, and M Takahashi, and K Matsumoto, and H Misaki, and T Ohshima
Novel bacterial peroxidase without catalase activity from Flavobacterium meningosepticum: purification and characterization.
November 1999, Biochimica et biophysica acta,
S Sakasegawa, and I Yoshioka, and S Koga, and M Takahashi, and K Matsumoto, and H Misaki, and T Ohshima
Chemical structure of lipid A isolated from Flavobacterium meningosepticum lipopolysaccharide.
August 1998, Journal of bacteriology,
S Sakasegawa, and I Yoshioka, and S Koga, and M Takahashi, and K Matsumoto, and H Misaki, and T Ohshima
Molecular cloning and heterologous expression of N-glycosidase F from Flavobacterium meningosepticum.
September 1990, The Journal of biological chemistry,
S Sakasegawa, and I Yoshioka, and S Koga, and M Takahashi, and K Matsumoto, and H Misaki, and T Ohshima
Cloning, sequencing, and expression of the dipeptidyl peptidase IV gene from Flavobacterium meningosepticum in Escherichia coli.
June 1995, Archives of biochemistry and biophysics,
S Sakasegawa, and I Yoshioka, and S Koga, and M Takahashi, and K Matsumoto, and H Misaki, and T Ohshima
Flavobacterium meningosepticum from neonatal meningitis.
May 1970, Japanese journal of microbiology,
S Sakasegawa, and I Yoshioka, and S Koga, and M Takahashi, and K Matsumoto, and H Misaki, and T Ohshima
Prolyl aminopeptidase gene from Flavobacterium meningosepticum: cloning, purification of the expressed enzyme, and analysis of its sequence.
December 1996, Archives of biochemistry and biophysics,
S Sakasegawa, and I Yoshioka, and S Koga, and M Takahashi, and K Matsumoto, and H Misaki, and T Ohshima
Thermostable glycerol kinase from a hyperthermophilic archaeon: gene cloning and characterization of the recombinant enzyme.
December 1998, Protein engineering,
S Sakasegawa, and I Yoshioka, and S Koga, and M Takahashi, and K Matsumoto, and H Misaki, and T Ohshima
The three-dimensional structure of PNGase F, a glycosylasparaginase from Flavobacterium meningosepticum.
November 1994, Structure (London, England : 1993),
Copied contents to your clipboard!