BIOMAT Database Logo BIOMAT Database Logo

hsp65 sequencing for identification of rapidly growing mycobacteria. 1999

H Ringuet, and C Akoua-Koffi, and S Honore, and A Varnerot, and V Vincent, and P Berche, and J L Gaillard, and C Pierre-Audigier
Service de Microbiologie, Hôpital Necker-Enfants Malades, 75015 Paris, France.

Partial sequencing of the hsp65 gene was used for the identification of rapidly growing mycobacteria (RGM). A 441-bp fragment (A. Telenti, F. Marchesi, M. Balz, F. Bally, E. Böttger, and T. Bodmer, J. Clin. Microbiol. 31:175-178, 1993) was amplified and sequenced by an automated fluorescence-based method involving capillary electrophoresis. Type strains of 10 RGM species were first studied. Each species had a unique nucleotide sequence, distinguishing it clearly from the other species. A panel of strains from the four main RGM species responsible for human infections, Mycobacterium abscessus, Mycobacterium chelonae, Mycobacterium fortuitum, and Mycobacterium peregrinum, was also studied. There were few sequence differences within each of these species (<2% of bases were different from the type strain sequence), and they had no effect on species assignment. hsp65 sequencing unambiguously differentiated M. chelonae and M. abscessus, two species difficult to identify by classical methods and 16S rRNA gene sequencing. The devised procedure is a rapid and reliable tool for the identification of RGM species.

UI MeSH Term Description Entries
D008969 Molecular Sequence Data Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories. Sequence Data, Molecular,Molecular Sequencing Data,Data, Molecular Sequence,Data, Molecular Sequencing,Sequencing Data, Molecular
D009161 Mycobacterium A genus of gram-positive, aerobic bacteria. Most species are free-living in soil and water, but the major habitat for some is the diseased tissue of warm-blooded hosts. Mycobacteria
D009164 Mycobacterium Infections Infections with bacteria of the genus MYCOBACTERIUM. Infections, Mycobacterium,Infection, Mycobacterium,Mycobacterium Infection
D009169 Mycobacterium tuberculosis A species of gram-positive, aerobic bacteria that produces TUBERCULOSIS in humans, other primates, CATTLE; DOGS; and some other animals which have contact with humans. Growth tends to be in serpentine, cordlike masses in which the bacilli show a parallel orientation. Mycobacterium tuberculosis H37Rv
D010802 Phylogeny The relationships of groups of organisms as reflected by their genetic makeup. Community Phylogenetics,Molecular Phylogenetics,Phylogenetic Analyses,Phylogenetic Analysis,Phylogenetic Clustering,Phylogenetic Comparative Analysis,Phylogenetic Comparative Methods,Phylogenetic Distance,Phylogenetic Generalized Least Squares,Phylogenetic Groups,Phylogenetic Incongruence,Phylogenetic Inference,Phylogenetic Networks,Phylogenetic Reconstruction,Phylogenetic Relatedness,Phylogenetic Relationships,Phylogenetic Signal,Phylogenetic Structure,Phylogenetic Tree,Phylogenetic Trees,Phylogenomics,Analyse, Phylogenetic,Analysis, Phylogenetic,Analysis, Phylogenetic Comparative,Clustering, Phylogenetic,Community Phylogenetic,Comparative Analysis, Phylogenetic,Comparative Method, Phylogenetic,Distance, Phylogenetic,Group, Phylogenetic,Incongruence, Phylogenetic,Inference, Phylogenetic,Method, Phylogenetic Comparative,Molecular Phylogenetic,Network, Phylogenetic,Phylogenetic Analyse,Phylogenetic Clusterings,Phylogenetic Comparative Analyses,Phylogenetic Comparative Method,Phylogenetic Distances,Phylogenetic Group,Phylogenetic Incongruences,Phylogenetic Inferences,Phylogenetic Network,Phylogenetic Reconstructions,Phylogenetic Relatednesses,Phylogenetic Relationship,Phylogenetic Signals,Phylogenetic Structures,Phylogenetic, Community,Phylogenetic, Molecular,Phylogenies,Phylogenomic,Reconstruction, Phylogenetic,Relatedness, Phylogenetic,Relationship, Phylogenetic,Signal, Phylogenetic,Structure, Phylogenetic,Tree, Phylogenetic
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000942 Antigens, Bacterial Substances elaborated by bacteria that have antigenic activity. Bacterial Antigen,Bacterial Antigens,Antigen, Bacterial
D001426 Bacterial Proteins Proteins found in any species of bacterium. Bacterial Gene Products,Bacterial Gene Proteins,Gene Products, Bacterial,Bacterial Gene Product,Bacterial Gene Protein,Bacterial Protein,Gene Product, Bacterial,Gene Protein, Bacterial,Gene Proteins, Bacterial,Protein, Bacterial,Proteins, Bacterial
D001483 Base Sequence The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence. DNA Sequence,Nucleotide Sequence,RNA Sequence,DNA Sequences,Base Sequences,Nucleotide Sequences,RNA Sequences,Sequence, Base,Sequence, DNA,Sequence, Nucleotide,Sequence, RNA,Sequences, Base,Sequences, DNA,Sequences, Nucleotide,Sequences, RNA
D012329 RNA, Bacterial Ribonucleic acid in bacteria having regulatory and catalytic roles as well as involvement in protein synthesis. Bacterial RNA

Related Publications

H Ringuet, and C Akoua-Koffi, and S Honore, and A Varnerot, and V Vincent, and P Berche, and J L Gaillard, and C Pierre-Audigier
Rapidly Growing Mycobacteria.
January 2017, Microbiology spectrum,
H Ringuet, and C Akoua-Koffi, and S Honore, and A Varnerot, and V Vincent, and P Berche, and J L Gaillard, and C Pierre-Audigier
Evaluation of INNO-LiPA mycobacteria v2 assay for identification of rapidly growing mycobacteria.
July 2011, Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology],
H Ringuet, and C Akoua-Koffi, and S Honore, and A Varnerot, and V Vincent, and P Berche, and J L Gaillard, and C Pierre-Audigier
A simple identification system for rapidly growing mycobacteria (a note).
January 1988, Acta microbiologica Hungarica,
H Ringuet, and C Akoua-Koffi, and S Honore, and A Varnerot, and V Vincent, and P Berche, and J L Gaillard, and C Pierre-Audigier
[Identification of acid-fast bacteria. 3. Rapidly growing mycobacteria].
September 1966, Igaku to seibutsugaku. Medicine and biology,
H Ringuet, and C Akoua-Koffi, and S Honore, and A Varnerot, and V Vincent, and P Berche, and J L Gaillard, and C Pierre-Audigier
Classification of rapidly growing mycobacteria.
June 1968, Japanese journal of microbiology,
H Ringuet, and C Akoua-Koffi, and S Honore, and A Varnerot, and V Vincent, and P Berche, and J L Gaillard, and C Pierre-Audigier
Taxonomy of rapidly growing mycobacteria.
January 1994, Clinical infectious diseases : an official publication of the Infectious Diseases Society of America,
H Ringuet, and C Akoua-Koffi, and S Honore, and A Varnerot, and V Vincent, and P Berche, and J L Gaillard, and C Pierre-Audigier
Characterization of rapidly growing mycobacteria using a commercial identification system.
January 2000, European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology,
H Ringuet, and C Akoua-Koffi, and S Honore, and A Varnerot, and V Vincent, and P Berche, and J L Gaillard, and C Pierre-Audigier
[Evaluation of INNO-LiPA mycobacteria, INNO-LiPA Mycobacteria v2 and hsp65 sequencing for identification of the clinical nontuberculous mycobacterial isolates].
October 2005, Mikrobiyoloji bulteni,
H Ringuet, and C Akoua-Koffi, and S Honore, and A Varnerot, and V Vincent, and P Berche, and J L Gaillard, and C Pierre-Audigier
rpoB-based identification of nonpigmented and late-pigmenting rapidly growing mycobacteria.
December 2003, Journal of clinical microbiology,
H Ringuet, and C Akoua-Koffi, and S Honore, and A Varnerot, and V Vincent, and P Berche, and J L Gaillard, and C Pierre-Audigier
[Rapidly growing mycobacteria in human pathology].
January 2000, Enfermedades infecciosas y microbiologia clinica,
Copied contents to your clipboard!