Elution of filtered granulocytes for transfusion. 1976

E E Morse, and A J Katz, and R F Reiss, and J L Houx

Functional and clinical transfusion studies were carried out on granulocytes eluted from nylon filters using ACD Saline solutions and 20% CPD Plasma ACD Saline solutions. Although the average volume of blood and total number of granulocytes filtered were the same, the yield of granulocytes eluted form the filters was 20% greater when 20% CPD plasma was in the eluting solution. Function studies indicated that while the antibacterial capacity of the saline eluted granulocytes was equivalent to that of plasma eluted cells, chemotactic activity was markedly reduced after 24 hours storage in the saline eluted cells. Clinical studies post transfusion also suggest better response from plasma eluted granulocytes.

UI MeSH Term Description Entries
D007962 Leukocytes White blood cells. These include granular leukocytes (BASOPHILS; EOSINOPHILS; and NEUTROPHILS) as well as non-granular leukocytes (LYMPHOCYTES and MONOCYTES). Blood Cells, White,Blood Corpuscles, White,White Blood Cells,White Blood Corpuscles,Blood Cell, White,Blood Corpuscle, White,Corpuscle, White Blood,Corpuscles, White Blood,Leukocyte,White Blood Cell,White Blood Corpuscle
D001770 Blood Bactericidal Activity The natural bactericidal property of BLOOD due to normally occurring antibacterial substances such as beta lysin, leukin, etc. This activity needs to be distinguished from the bactericidal activity contained in a patient's serum as a result of antimicrobial therapy, which is measured by a SERUM BACTERICIDAL TEST. Activities, Blood Bactericidal,Activity, Blood Bactericidal,Bactericidal Activities, Blood,Bactericidal Activity, Blood,Blood Bactericidal Activities
D001803 Blood Transfusion The introduction of whole blood or blood component directly into the blood stream. (Dorland, 27th ed) Blood Transfusions,Transfusion, Blood,Transfusions, Blood
D002634 Chemotaxis, Leukocyte The movement of leukocytes in response to a chemical concentration gradient or to products formed in an immunologic reaction. Leukotaxis,Leukocyte Chemotaxis
D005374 Filtration A process of separating particulate matter from a fluid, such as air or a liquid, by passing the fluid carrier through a medium that will not pass the particulates. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed) Filtrations
D006098 Granulocytes Leukocytes with abundant granules in the cytoplasm. They are divided into three groups according to the staining properties of the granules: neutrophilic, eosinophilic, and basophilic. Mature granulocytes are the NEUTROPHILS; EOSINOPHILS; and BASOPHILS. Granulocyte
D012462 Saline Solution, Hypertonic Hypertonic sodium chloride solution. A solution having an osmotic pressure greater than that of physiologic salt solution (0.9 g NaCl in 100 ml purified water). Hypertonic Saline Solution,Hypertonic Solution, Saline,Sodium Chloride Solution, Hypertonic,Hypertonic Saline Solutions,Hypertonic Solutions, Saline,Saline Solutions, Hypertonic,Sodium Chloride Solutions, Hypertonic,Saline Hypertonic Solution,Saline Hypertonic Solutions,Solution, Hypertonic Saline,Solution, Saline Hypertonic,Solutions, Hypertonic Saline,Solutions, Saline Hypertonic

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