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A fluorimetric method for determination of rubomycin in the blood, milk and urine of humans and animals in concentrations of 0.04-20 gamma/ml was developed. After protein precipitation from the biological material with trichloracetic acid and the antibiotic was determined by fluorescence of its borate complex in concentrated sulfuric acid (gammaexcitk = 575, gammaflk = 620 nm).
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