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A novel method has been developed for the study of phospholipid exchange and fusion of phospholipid vesicles. Two homogeneous populations of single bilayer phosphatidylcholine vesicles of similar size but markedly different density have been prepared. "Dense" vesicles were made from brominated dioleoyl phosphatidylcholine. "Light" vesicles were prepared from dioleoyl phosphatidylcholine. The two populations were easily separated by density gradient centrifugation. Phosphatidylcholine exchange protein from beef liver was used to promote lecithin exchange between the vesicle populations. Only the lecithin of the external monolayers of the vesicles was available for exchange by exchange protein, implying that flip-flop of vesicle phosphatidylcholine did not take place at a detectable frequency. No spontaneous intervesicle phosphatidylcholine exchange was observed. However, the dense and light vesicles did spontaneously fuse, over several hours, to produce particles of hybrid density.
E A Dawidowicz, and
J E Rothman
May 1975,
Biochemistry,
E A Dawidowicz, and
J E Rothman
February 1981,
Biochimica et biophysica acta,
E A Dawidowicz, and
J E Rothman
January 1976,
Biochemistry,
E A Dawidowicz, and
J E Rothman
May 1979,
Biochemistry,
E A Dawidowicz, and
J E Rothman
November 1975,
Biochemistry,
E A Dawidowicz, and
J E Rothman
July 1978,
The Journal of membrane biology,
E A Dawidowicz, and
J E Rothman
October 1978,
The Journal of biological chemistry,
E A Dawidowicz, and
J E Rothman
March 1977,
FEBS letters,
E A Dawidowicz, and
J E Rothman
August 1978,
Biochemistry,
E A Dawidowicz, and
J E Rothman
August 1982,
Biochimica et biophysica acta,
