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Production of specific monoclonal antibodies to Aspergillus species and their use in immunohistochemical identification of aspergillosis. 1999
L E Fenelon, and
A J Hamilton, and
J I Figueroa, and
M A Bartholomew, and
M H Allen, and
P McCarthy, and
R J Hay
Departments of Clinical Microbiology and Histopathology, St. Vincent's Hospital, Dublin 4, Ireland. lfenelon@svherc.ucd.ie
Two anti-Aspergillus murine monoclonal antibodies (MAbs), designated 164G and 611F, have been produced; both specifically recognize cytoplasmic antigens of A. fumigatus, A. flavus, and A. niger by enzyme-linked immunosorbent assay. The MAbs can identify Aspergillus spp. both in frozen sections by immunofluorescence and in paraffin-embedded clinical specimens by immunofluorescence and immunoperoxidase staining.
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