Biomaterial Database

Glutamate receptor 5/6/7-like and glutamate transporter-1-like immunoreactivity in the leech central nervous system. 1999

M S Thorogood, and V W Almeida, and P D Brodfuehrer

Biology Department, Bryn Mawr College, Pennsylvania 19010, USA.

Previous physiological and pharmacological evidence has suggested a neurotransmitter role for the excitatory amino acid glutamate in the leech central nervous system (CNS). In the present study, we sought to localize glutamate receptor (GluR) subunits (GluR 5/6/7, GluR 2/3 and N-methyl-D-aspartate receptor 1 [NMDAR 1]) and a glutamate transporter subtype [GLT-1] within the leech CNS using mono- and polyclonal antibodies. In whole-mounted tissue, small cells of the outer capsule and putative microglia labeled with both GluR 5/6/7 and GluR 2/3 but not NMDAR 1 subunit antisera. In general, GluR 5/6/7-like immunofluorescence was both more intense and more widespread than GluR 2/3-like immunolabeling. Cryostat-sectioned tissue revealed extensive GluR 5/6/7-like immunoreactivity throughout the neuropil as well as labeling within a few neuronal somata. GLT-1-like immunoreactivity localized to the inner capsule, which is the interface between neuronal somata and the neuropil and is deeply invested by processes of neuropil glia. These results complement previous physiological and pharmacological findings indicating that the leech CNS possesses the cellular machinery to respond to glutamate and to transport glutamate from extracellular spaces. Together, they provide further evidence for glutamate's role as a neurotransmitter within the leech CNS.

UI	MeSH Term	Description	Entries
D007150	Immunohistochemistry	Histochemical localization of immunoreactive substances using labeled antibodies as reagents.	Immunocytochemistry,Immunogold Techniques,Immunogold-Silver Techniques,Immunohistocytochemistry,Immunolabeling Techniques,Immunogold Technics,Immunogold-Silver Technics,Immunolabeling Technics,Immunogold Silver Technics,Immunogold Silver Techniques,Immunogold Technic,Immunogold Technique,Immunogold-Silver Technic,Immunogold-Silver Technique,Immunolabeling Technic,Immunolabeling Technique,Technic, Immunogold,Technic, Immunogold-Silver,Technic, Immunolabeling,Technics, Immunogold,Technics, Immunogold-Silver,Technics, Immunolabeling,Technique, Immunogold,Technique, Immunogold-Silver,Technique, Immunolabeling,Techniques, Immunogold,Techniques, Immunogold-Silver,Techniques, Immunolabeling
D007865	Leeches	Annelids of the class Hirudinea. Some species, the bloodsuckers, may become temporarily parasitic upon animals, including man. Medicinal leeches (HIRUDO MEDICINALIS) have been used therapeutically for drawing blood since ancient times.	Hirudinea,Hirudineas,Leeche
D009004	Monosaccharide Transport Proteins	A large group of membrane transport proteins that shuttle MONOSACCHARIDES across CELL MEMBRANES.	Hexose Transport Proteins,Band 4.5 Preactin,Erythrocyte Band 4.5 Protein,Glucose Transport-Inducing Protein,Hexose Transporter,4.5 Preactin, Band,Glucose Transport Inducing Protein,Preactin, Band 4.5,Proteins, Monosaccharide Transport,Transport Proteins, Hexose,Transport Proteins, Monosaccharide,Transport-Inducing Protein, Glucose
D002490	Central Nervous System	The main information-processing organs of the nervous system, consisting of the brain, spinal cord, and meninges.	Cerebrospinal Axis,Axi, Cerebrospinal,Axis, Cerebrospinal,Central Nervous Systems,Cerebrospinal Axi,Nervous System, Central,Nervous Systems, Central,Systems, Central Nervous
D000097806	GluK2 Kainate Receptor	A high-affinity KAINATE and GLUTAMATE receptor that functions as ligand-gated ion channel in the CENTRAL NERVOUS SYSYEM and plays an essential role in NEURONAL PLASTICITY.	GluR6 Kainate Receptor,GluR6 Kainate Receptors,GluR6 Receptor,Ionotropic Glutamate Receptor GluR6,Receptor, GluK2 Kainate,Receptor, GluR6,Receptor, GluR6 Kainate,Receptors, GluR6 Kainate
D000097808	GluK3 Kainate Receptor	A high-affinity KAINATE and GLUTAMATE receptor that functions as ligand-gated ion channel in the CENTRAL NERVOUS SYSYEM and modulates excitatory synaptic transmission.	EAA5 Receptor,EAA5 Receptors,GluR7 Receptor,Receptor, EAA5,Receptor, GluK3 Kainate,Receptor, GluR7
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D051272	Glucose Transporter Type 1	A ubiquitously expressed glucose transporter that is important for constitutive, basal GLUCOSE transport. It is predominately expressed in ENDOTHELIAL CELLS and ERYTHROCYTES at the BLOOD-BRAIN BARRIER and is responsible for GLUCOSE entry into the BRAIN.	Erythrocyte Glucose Transporter,GLUT-1 Protein,GLUT1 Protein,SLC2A1 Protein,Solute Carrier Family 2, Facilitated Glucose Transporter, Member 1 Protein,GLUT 1 Protein,Glucose Transporter, Erythrocyte
D017952	Ganglia, Invertebrate	Clusters of neuronal cell bodies in invertebrates. Invertebrate ganglia may also contain neuronal processes and non-neuronal supporting cells. Many invertebrate ganglia are favorable subjects for research because they have small numbers of functional neuronal types which can be identified from one animal to another.	Invertebrate Ganglia,Ganglion, Invertebrate,Ganglions, Invertebrate,Invertebrate Ganglion,Invertebrate Ganglions
D018092	Receptors, Kainic Acid	A class of ionotropic glutamate receptors characterized by their affinity for KAINIC ACID.	Kainate Receptors,Kainic Acid Receptors,Receptors, Kainate,Kainate Receptor,Kainic Acid Receptor,Receptor, Kainate,Receptor, Kainic Acid