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Efflux of taurocholate from isolated rat hepatocytes was studied to characterize the mechanism of bile acid secretion. Cells were incubated with taurocholate for 15 min. The amount of the intracellularly accumulated bile acid was directly related to the concentration in the medium. Transfer of the loaded cells from the incubation medium to a medium without taurocholate led to taurocholate efflux. Efflux was saturable, its activation energy amounted to 12 kcal/mol (50 kJ). It was strongly inhibited by the metabolic inhibitor antimycin A and to a lesser extend by the uncoupler carbonylcyanide-m-chlorophenylhydrazone. Dinitrofluorobenzene and mersalyl, reagents which react with amino acids, inhibited efflux by about 30% when applied at concentrations of 50 muM. Ouabain increased the rate of efflux. The observations indicate that secretory functions are maintained in isolated liver cells.
L R Schwarz, and
M Schwenk, and
E Pfaff, and
H Greim
April 1982,
Pediatric research,
L R Schwarz, and
M Schwenk, and
E Pfaff, and
H Greim
January 1985,
Biology of the neonate,
L R Schwarz, and
M Schwenk, and
E Pfaff, and
H Greim
April 1987,
The American journal of physiology,
L R Schwarz, and
M Schwenk, and
E Pfaff, and
H Greim
January 1987,
Hepatology (Baltimore, Md.),
L R Schwarz, and
M Schwenk, and
E Pfaff, and
H Greim
October 1984,
Gastroenterology,
L R Schwarz, and
M Schwenk, and
E Pfaff, and
H Greim
November 1979,
Research communications in chemical pathology and pharmacology,
L R Schwarz, and
M Schwenk, and
E Pfaff, and
H Greim
November 1988,
Biochemical pharmacology,
L R Schwarz, and
M Schwenk, and
E Pfaff, and
H Greim
February 2005,
Toxicological sciences : an official journal of the Society of Toxicology,
L R Schwarz, and
M Schwenk, and
E Pfaff, and
H Greim
May 1990,
Hepatology (Baltimore, Md.),
L R Schwarz, and
M Schwenk, and
E Pfaff, and
H Greim
October 1993,
The Journal of pharmacology and experimental therapeutics,
