Biomaterial Database

Theileria annulata: genetic characterization of Spanish isolates by isoenzyme electrophoresis and random amplified polymorphic DNA. 1999

J Martin-Sanchez, and P Garcia-Fernandez

Departamento de Producción Animal, Centro de Investigación y Formación Agraría, Camino de Purchil s/n, 18.071 Granada, Spain.

Isoenzyme electrophoresis and RAPD techniques were used to study the genetic polymorphism of different Spanish isolates of Theileria annulata in the schizont and piroplasm stages. Enzyme activity attributable to the parasite was detected in only 5 of the 13 loci studied with isoenzyme electrophoresis. Of these, differences between the cell lines studied were found only in the loci GPI, ICD, and FH. Only 6 of the 11 primers used in the RAPD generated reproducible genomic DNA fingerprints. None of the amplification products generated using primers ILO 509, ILO 525, ILO 872, and ILO 875 hybridized with DNA of the bovine cell line not infected by T. annulata, BL20, indicating that this technique can be used with either piroplasm DNA or DNA from parasite schizonts after first passing it through DEAE cellulose columns. The results obtained with both characterization techniques demonstrate a moderate degree of polymorphism among the Spanish isolates of this protozoan.

UI	MeSH Term	Description	Entries
D007527	Isoenzymes	Structurally related forms of an enzyme. Each isoenzyme has the same mechanism and classification, but differs in its chemical, physical, or immunological characteristics.	Alloenzyme,Allozyme,Isoenzyme,Isozyme,Isozymes,Alloenzymes,Allozymes
D011110	Polymorphism, Genetic	The regular and simultaneous occurrence in a single interbreeding population of two or more discontinuous genotypes. The concept includes differences in genotypes ranging in size from a single nucleotide site (POLYMORPHISM, SINGLE NUCLEOTIDE) to large nucleotide sequences visible at a chromosomal level.	Gene Polymorphism,Genetic Polymorphism,Polymorphism (Genetics),Genetic Polymorphisms,Gene Polymorphisms,Polymorphism, Gene,Polymorphisms (Genetics),Polymorphisms, Gene,Polymorphisms, Genetic
D002417	Cattle	Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.	Beef Cow,Bos grunniens,Bos indicus,Bos indicus Cattle,Bos taurus,Cow,Cow, Domestic,Dairy Cow,Holstein Cow,Indicine Cattle,Taurine Cattle,Taurus Cattle,Yak,Zebu,Beef Cows,Bos indicus Cattles,Cattle, Bos indicus,Cattle, Indicine,Cattle, Taurine,Cattle, Taurus,Cattles, Bos indicus,Cattles, Indicine,Cattles, Taurine,Cattles, Taurus,Cow, Beef,Cow, Dairy,Cow, Holstein,Cows,Dairy Cows,Domestic Cow,Domestic Cows,Indicine Cattles,Taurine Cattles,Taurus Cattles,Yaks,Zebus
D004587	Electrophoresis, Agar Gel	Electrophoresis in which agar or agarose gel is used as the diffusion medium.	Electrophoresis, Agarose Gel,Agar Gel Electrophoresis,Agarose Gel Electrophoresis,Gel Electrophoresis, Agar,Gel Electrophoresis, Agarose
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D013030	Spain	Country located between France on the northeast and Portugal on the west and bordered by the Atlantic Ocean and the Mediterranean Sea. The capital is Madrid.	Balearic Islands,Canary Islands
D013801	Theileriasis	Infection of cattle, sheep, or goats with protozoa of the genus THEILERIA. This infection results in an acute or chronic febrile condition.	Corridor Disease,East Coast Fever,Theileriosis,Coast Fever, East,Disease, Corridor,Fever, East Coast,Theileriases,Theilerioses
D016054	DNA, Protozoan	Deoxyribonucleic acid that makes up the genetic material of protozoa.	Protozoan DNA
D016796	Theileria annulata	A protozoan parasite causing tropical theileriasis in cattle. It is transmitted by ticks of the Hyalomma genus.	Theileria annulatas,annulatas, Theileria
D019105	Random Amplified Polymorphic DNA Technique	Technique that utilizes low-stringency polymerase chain reaction (PCR) amplification with single primers of arbitrary sequence to generate strain-specific arrays of anonymous DNA fragments. RAPD technique may be used to determine taxonomic identity, assess kinship relationships, analyze mixed genome samples, and create specific probes.	RAPD Technique,RAPD-PCR,Arbitrarily Primed PCR Reaction,RAPD Technic,Random Amplified Polymorphic DNA Technic,RAPD Technics,RAPD Techniques