Molecular characterization of Brazilian human Giardia duodenalis isolates using isoenzyme and random amplified polymorphic DNA analysis. 2003

Míriam O Rocha, and Maria A Gomes, and Adriana O Costa, and Cinthia Furst, and Edward F Silva
School of Pharmacy, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil. morocha@farmacia.ufmg.br

Isoenzymes and RAPD (random amplified polymorphic DNA) analysis were used to characterize three Brazilian human isolates of Giardia duodenalis and its clones. The Portland-1 strain (ATCC 30888) was included in the study as a reference pattern. Both methods divided the isolates into two main groups, one represented by the Portland-1 strain, the other constituted by the Brazilian isolates, which, in turn, were divided into 2 subgroups. The dendogram constructed with the RAPD data, using seven primers, revealed a great heterogeneity between Brazilian isolates and the Portland-1 strain. There was no relationship to the clinical characteristics of the isolates. Although a lot of similarity has been observed among Brazilian isolates and its clones, individual polymorphism was detected, which could be related to the clonal reproduction of this protozoan.

UI MeSH Term Description Entries
D007527 Isoenzymes Structurally related forms of an enzyme. Each isoenzyme has the same mechanism and classification, but differs in its chemical, physical, or immunological characteristics. Alloenzyme,Allozyme,Isoenzyme,Isozyme,Isozymes,Alloenzymes,Allozymes
D008297 Male Males
D008969 Molecular Sequence Data Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories. Sequence Data, Molecular,Molecular Sequencing Data,Data, Molecular Sequence,Data, Molecular Sequencing,Sequencing Data, Molecular
D001938 Brazil A country located on the eastern coast of South America, located between Colombia and Peru, that borders the Atlantic Ocean. It is bordered on the north by Venezuela, Guyana, Suriname, and French Guiana, on the south by Uruguay, and on the west by Argentina. The capital is Brasilia.
D004592 Electrophoresis, Starch Gel Electrophoresis in which a starch gel (a mixture of amylose and amylopectin) is used as the diffusion medium. Starch Gel Electrophoresis
D005260 Female Females
D005873 Giardiasis An infection of the SMALL INTESTINE caused by the flagellated protozoan GIARDIA. It is spread via contaminated food and water and by direct person-to-person contact. Lambliasis,Giardia Infection,Giardia duodenalis Infection,Giardia intestinalis Infection,Giardia lamblia Infection,Giardia Infections,Giardia duodenalis Infections,Giardia intestinalis Infections,Giardia lamblia Infections,Giardiases,Infection, Giardia,Infection, Giardia duodenalis,Infection, Giardia intestinalis,Infection, Giardia lamblia,Infections, Giardia,Infections, Giardia duodenalis,Infections, Giardia intestinalis,Infections, Giardia lamblia,Lambliases
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D001483 Base Sequence The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence. DNA Sequence,Nucleotide Sequence,RNA Sequence,DNA Sequences,Base Sequences,Nucleotide Sequences,RNA Sequences,Sequence, Base,Sequence, DNA,Sequence, Nucleotide,Sequence, RNA,Sequences, Base,Sequences, DNA,Sequences, Nucleotide,Sequences, RNA

Related Publications

Míriam O Rocha, and Maria A Gomes, and Adriana O Costa, and Cinthia Furst, and Edward F Silva
January 1993, Transactions of the Royal Society of Tropical Medicine and Hygiene,
Míriam O Rocha, and Maria A Gomes, and Adriana O Costa, and Cinthia Furst, and Edward F Silva
June 1995, The American journal of tropical medicine and hygiene,
Míriam O Rocha, and Maria A Gomes, and Adriana O Costa, and Cinthia Furst, and Edward F Silva
June 1999, Parasitology research,
Míriam O Rocha, and Maria A Gomes, and Adriana O Costa, and Cinthia Furst, and Edward F Silva
May 1999, Experimental parasitology,
Míriam O Rocha, and Maria A Gomes, and Adriana O Costa, and Cinthia Furst, and Edward F Silva
February 1995, Journal of clinical microbiology,
Míriam O Rocha, and Maria A Gomes, and Adriana O Costa, and Cinthia Furst, and Edward F Silva
March 2002, Revista iberoamericana de micologia,
Míriam O Rocha, and Maria A Gomes, and Adriana O Costa, and Cinthia Furst, and Edward F Silva
May 2007, Acta tropica,
Míriam O Rocha, and Maria A Gomes, and Adriana O Costa, and Cinthia Furst, and Edward F Silva
June 1997, Japanese journal of medical science & biology,
Míriam O Rocha, and Maria A Gomes, and Adriana O Costa, and Cinthia Furst, and Edward F Silva
August 1999, Journal of microbiological methods,
Míriam O Rocha, and Maria A Gomes, and Adriana O Costa, and Cinthia Furst, and Edward F Silva
January 2003, Experimental parasitology,
Copied contents to your clipboard!