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Caffeine was analyzed in human plasma and saliva by a simple, rapid, and sensitive radioimmunoassay procedure. Immunization of rabbits with an antigen prepared by coupling 7-(5-carboxypentyl)-1,3-dimethylxanthine to bovine serum albumin resulted in the formation of antibodies selective for caffeine as opposed to various mono- and dimethylxanthines, mono-, di-, and trimethyluric acids and a variety of common drugs. The radioligand used for competitive binding studies was 7-(2,3-3H2-propyl)-1,3-dimethylxanthine. The procedure permits direct analysis of caffeine in plasma or saliva without extraction. Comparison with a high pressure liquid chromatography method for the analysis of caffeine gave satisfactory results and showed no evidence for interference by metabolites. A caffeine half-life of 4.0 hours determined by the radioimmunoassay was in agreement with previous work. Comparison of human plasma and saliva levels by the radioimmunoassay procedure indicated approximately equal concentrations in the two fluids.
C E Cook, and
C R Tallent, and
E W Amerson, and
M W Myers, and
J A Kepler, and
G F Taylor, and
H D Christensen
February 1980,
Research communications in chemical pathology and pharmacology,
C E Cook, and
C R Tallent, and
E W Amerson, and
M W Myers, and
J A Kepler, and
G F Taylor, and
H D Christensen
October 1982,
Journal of clinical chemistry and clinical biochemistry. Zeitschrift fur klinische Chemie und klinische Biochemie,
C E Cook, and
C R Tallent, and
E W Amerson, and
M W Myers, and
J A Kepler, and
G F Taylor, and
H D Christensen
December 1975,
Clinical pharmacology and therapeutics,
C E Cook, and
C R Tallent, and
E W Amerson, and
M W Myers, and
J A Kepler, and
G F Taylor, and
H D Christensen
July 1984,
Clinical pharmacology and therapeutics,
C E Cook, and
C R Tallent, and
E W Amerson, and
M W Myers, and
J A Kepler, and
G F Taylor, and
H D Christensen
July 1988,
Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan,
C E Cook, and
C R Tallent, and
E W Amerson, and
M W Myers, and
J A Kepler, and
G F Taylor, and
H D Christensen
September 1982,
Annals of clinical biochemistry,
C E Cook, and
C R Tallent, and
E W Amerson, and
M W Myers, and
J A Kepler, and
G F Taylor, and
H D Christensen
January 1978,
Clinical chemistry,
C E Cook, and
C R Tallent, and
E W Amerson, and
M W Myers, and
J A Kepler, and
G F Taylor, and
H D Christensen
January 1986,
Cancer chemotherapy and pharmacology,
C E Cook, and
C R Tallent, and
E W Amerson, and
M W Myers, and
J A Kepler, and
G F Taylor, and
H D Christensen
December 1975,
European journal of clinical pharmacology,
C E Cook, and
C R Tallent, and
E W Amerson, and
M W Myers, and
J A Kepler, and
G F Taylor, and
H D Christensen
August 1978,
Lijecnicki vjesnik,
