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A radioimmunoassay method for the assay of luteinising hormone (LH) and follicle-stimulating hormone (FSH) in serum/plasma has been designed for use in laboratories of varying expertise in the United Kingdom. The major sources of experimental error leading to poor within-laboratory performance and between-laboratory comparability were identified: quality of tracer, use of calibration standards, and separation procedure. A simple rugged kit was designed which was extensively tested first in our laboratory and then in a small multi-centre field trial before being made available. It is now used routinely by 26 health service and research laboratories. The working range of the assays is 1-50 IU/l (LH) and 0.3-16 IU/l (FSH). The between-batch reproducibility was 5-11% (CV) over the dose range 4.8-18 IU/l (LH) and 1.6-15 IU/l (FSH).
K M Ferguson, and
M Hayes, and
S L Jeffcoate
January 1965,
Nature,
K M Ferguson, and
M Hayes, and
S L Jeffcoate
January 1986,
Cancer chemotherapy and pharmacology,
K M Ferguson, and
M Hayes, and
S L Jeffcoate
December 1975,
European journal of clinical pharmacology,
K M Ferguson, and
M Hayes, and
S L Jeffcoate
April 1982,
Medical laboratory sciences,
K M Ferguson, and
M Hayes, and
S L Jeffcoate
December 1976,
The Journal of pharmacology and experimental therapeutics,
K M Ferguson, and
M Hayes, and
S L Jeffcoate
December 1989,
Journal of pharmaceutical sciences,
K M Ferguson, and
M Hayes, and
S L Jeffcoate
January 1967,
The Journal of endocrinology,
K M Ferguson, and
M Hayes, and
S L Jeffcoate
January 1976,
Journal of immunological methods,
K M Ferguson, and
M Hayes, and
S L Jeffcoate
May 1966,
Lancet (London, England),
K M Ferguson, and
M Hayes, and
S L Jeffcoate
January 1981,
Annals of clinical biochemistry,
