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Nucleosome DNA repeat lengths in Physarum chromatin, determined by nuclease digestion experiments, are shorter than those observed in most mammalian chromatin and longer than those reported for chromatin of certain other lower eukaryotes. After digestion with staphylococcal nuclease for short periods of time an average repeat length of 190 base pairs is measured. After more extensive digestion an average repeat length of 172 base pairs is measured. Upon prolonged digestion DNA is degraded to an average monomer subunit length of 160 base pairs, with only a small amount of DNA found in lengths of 130 base pairs or smaller. Mathematical analysis of the data suggests that the Physarum nucleosome DNA repeat comprises a protected DNA segment of about 159 base pairs with a nuclease-accessible interconnecting segment which ranges from 13 to 31 base pairs. The spacing data are compatible with measurements from electron micrographs of Physarum chromatin.
E M Johnson, and
V C Littau, and
V G Allfrey, and
E M Bradbury, and
H R Matthews
March 1976,
FEBS letters,
E M Johnson, and
V C Littau, and
V G Allfrey, and
E M Bradbury, and
H R Matthews
March 1977,
Biochimica et biophysica acta,
E M Johnson, and
V C Littau, and
V G Allfrey, and
E M Bradbury, and
H R Matthews
March 1978,
Nucleic acids research,
E M Johnson, and
V C Littau, and
V G Allfrey, and
E M Bradbury, and
H R Matthews
June 1978,
FEBS letters,
E M Johnson, and
V C Littau, and
V G Allfrey, and
E M Bradbury, and
H R Matthews
August 1987,
Journal of molecular biology,
E M Johnson, and
V C Littau, and
V G Allfrey, and
E M Bradbury, and
H R Matthews
July 1975,
Tsitologiia,
E M Johnson, and
V C Littau, and
V G Allfrey, and
E M Bradbury, and
H R Matthews
August 1976,
Chromosoma,
E M Johnson, and
V C Littau, and
V G Allfrey, and
E M Bradbury, and
H R Matthews
August 1988,
The Journal of biological chemistry,
E M Johnson, and
V C Littau, and
V G Allfrey, and
E M Bradbury, and
H R Matthews
August 1988,
The Journal of biological chemistry,
E M Johnson, and
V C Littau, and
V G Allfrey, and
E M Bradbury, and
H R Matthews
January 1980,
Acta biochimica Polonica,
