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A rapid and efficient procedure is described for mapping and cloning the 5'-ends of mRNAs, including those generated from a unique gene by alternative splicing and promoter usage. This method involves reverse transcription of the targeted mRNAs from a long, highly labeled specific primer, resolution of the extension products on a DNA sequencing gel, elution and poly(dC) tailing of the single-stranded cDNAs of interest, amplification of these cDNAs by PCR using an oligo(dG) adapter-primer and a gene-specific primer and finally DNA sequencing of the subcloned PCR fragments. The overall method is called PEETA (primer extension, electrophoresis, elution, tailing and amplification).
G Flouriot, and
H Brand, and
F Gannon
September 1993,
Nucleic acids research,
G Flouriot, and
H Brand, and
F Gannon
October 2017,
Molecular & cellular proteomics : MCP,
G Flouriot, and
H Brand, and
F Gannon
December 2002,
Biochemical and biophysical research communications,
G Flouriot, and
H Brand, and
F Gannon
January 2020,
PloS one,
G Flouriot, and
H Brand, and
F Gannon
August 2001,
Memorias do Instituto Oswaldo Cruz,
G Flouriot, and
H Brand, and
F Gannon
October 1996,
Clinical and diagnostic virology,
G Flouriot, and
H Brand, and
F Gannon
February 2016,
IUBMB life,
G Flouriot, and
H Brand, and
F Gannon
March 2020,
Annals of translational medicine,
G Flouriot, and
H Brand, and
F Gannon
October 2017,
Reproductive sciences (Thousand Oaks, Calif.),
G Flouriot, and
H Brand, and
F Gannon
March 2002,
FEBS letters,
