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We have significantly improved a method originally developed by Liang and Pardee [Science 257 (1992) 967-971] to display a broad spectrum of expressed genes and to detect differences in expression between different cell types. We have analysed various aspects of the technique and have modified it for both, the application to fast and efficient identification of genes and the use with automatic analysis systems. Based on the mathematical background we have devised the appropriate number of optimal PCR primers. We have also introduced nondenaturating gels for separating double stranded fragments as single bands. By applying the method to regenerating mouse liver, we have identified, out of a total of 38,000 bands, about 70 fragments where the expression of the corresponding genes seems to be differentially regulated at different time points. Application of the method to an automatic DNA sequencer was successfully done. Thus, we have confirmed the usefulness and increased the power of the RNA display technique, which we named differential display reverse transcription PCR (DDRT-PCR), and have extended the range of its application.
D Bauer, and
H Müller, and
J Reich, and
H Riedel, and
V Ahrenkiel, and
P Warthoe, and
M Strauss
January 1997,
Methods in molecular biology (Clifton, N.J.),
D Bauer, and
H Müller, and
J Reich, and
H Riedel, and
V Ahrenkiel, and
P Warthoe, and
M Strauss
October 1994,
PCR methods and applications,
D Bauer, and
H Müller, and
J Reich, and
H Riedel, and
V Ahrenkiel, and
P Warthoe, and
M Strauss
August 1999,
Nucleic acids research,
D Bauer, and
H Müller, and
J Reich, and
H Riedel, and
V Ahrenkiel, and
P Warthoe, and
M Strauss
January 1999,
Methods in molecular medicine,
D Bauer, and
H Müller, and
J Reich, and
H Riedel, and
V Ahrenkiel, and
P Warthoe, and
M Strauss
August 1998,
Microbial pathogenesis,
D Bauer, and
H Müller, and
J Reich, and
H Riedel, and
V Ahrenkiel, and
P Warthoe, and
M Strauss
April 2019,
Bulletin of environmental contamination and toxicology,
D Bauer, and
H Müller, and
J Reich, and
H Riedel, and
V Ahrenkiel, and
P Warthoe, and
M Strauss
October 1996,
Clinical and diagnostic virology,
D Bauer, and
H Müller, and
J Reich, and
H Riedel, and
V Ahrenkiel, and
P Warthoe, and
M Strauss
December 1998,
Microbial pathogenesis,
D Bauer, and
H Müller, and
J Reich, and
H Riedel, and
V Ahrenkiel, and
P Warthoe, and
M Strauss
December 2002,
Biochemical and biophysical research communications,
D Bauer, and
H Müller, and
J Reich, and
H Riedel, and
V Ahrenkiel, and
P Warthoe, and
M Strauss
June 2018,
Research in veterinary science,
