Biomaterial Database

A new iridovirus isolated from soft-shelled turtle. 1999

Z X Chen, and J C Zheng, and Y L Jiang

Shenzhen Animal and Plant Quarantine Bureau, People's Republic of China.

A virus was isolated from soft-shelled turtle (Trionyx sinensis) with 'red neck disease' on a farm in Shenzhen, China, the virus multiplied and caused a cytopathogenic effect (CPE) at 15-30 degrees C in CO, FHM, CK and BF-2 cells. The optimum conditions for replication was in CO cells at 25-30 degrees C. The virus was sensitive to chloroform treatment, acid (pH 3) or alkaline (pH 10) conditions and heating at 56 degrees C for 30 min. Treatment with 5-iodo-2-deoxyuridine (IUDR) inhibited viral replication, indicating the presence of a DNA genome. Electron microscopy of infected CO culture fluid revealed spherical particles measuring 120-160 nm in diameter. Observation of ultra-thin sections showed numerous hexagonal viral particles in the cytoplasm and nucleus of cells typical of an iridovirus. This virus was moderately virulent for turtles in infection tests. We suggest that this virus is named soft-shelled turtle iridovirus (STIV).

UI	MeSH Term	Description	Entries
D007065	Idoxuridine	An analog of DEOXYURIDINE that inhibits viral DNA synthesis. The drug is used as an antiviral agent.	5-Iodo-2'-deoxyuridine,IUdR,Iododeoxyuridine,5-Iododeoxyuridine,Allergan 211,Herplex Liquifilm,Idoxuridine, 123I-Labeled,Idoxuridine, 125I-Labeled,Idoxuridine, 131I-Labeled,Idoxuridine, 3H-Labeled,Idoxuridine, Radical Ion (+1),Idoxuridine, Radical Ion (1-),Kerecide,NSC-39661,Oftan-IDU,SK&F-14287,Stoxil,123I-Labeled Idoxuridine,125I-Labeled Idoxuridine,131I-Labeled Idoxuridine,3H-Labeled Idoxuridine,5 Iodo 2' deoxyuridine,5 Iododeoxyuridine,Idoxuridine, 123I Labeled,Idoxuridine, 125I Labeled,Idoxuridine, 131I Labeled,Idoxuridine, 3H Labeled,Liquifilm, Herplex,NSC 39661,NSC39661,Oftan IDU,OftanIDU
D007497	Iridoviridae	A family of large icosahedral DNA viruses infecting insects and poikilothermic vertebrates. Genera include IRIDOVIRUS; RANAVIRUS; Chloriridovirus; Megalocytivirus; and Lymphocystivirus.	Chloriridovirus,Goldfish Viruses,Icosahedral Cytoplasmic Deoxyriboviruses,Lymphocystivirus,Megalocytivirus,Chloriridoviruses,Cytoplasmic Deoxyriboviruses, Icosahedral,Deoxyriboviruses, Icosahedral Cytoplasmic,Goldfish Virus,Lymphocystiviruses,Megalocytiviruses,Viruses, Goldfish
D002460	Cell Line	Established cell cultures that have the potential to propagate indefinitely.	Cell Lines,Line, Cell,Lines, Cell
D002681	China	A country spanning from central Asia to the Pacific Ocean.	Inner Mongolia,Manchuria,People's Republic of China,Sinkiang,Mainland China
D002725	Chloroform	A commonly used laboratory solvent. It was previously used as an anesthetic, but was banned from use in the U.S. due to its suspected carcinogenicity.	Trichloromethane
D004279	DNA, Viral	Deoxyribonucleic acid that makes up the genetic material of viruses.	Viral DNA
D005399	Fishes	A group of cold-blooded, aquatic vertebrates having gills, fins, a cartilaginous or bony endoskeleton, and elongated bodies covered with scales.
D006358	Hot Temperature	Presence of warmth or heat or a temperature notably higher than an accustomed norm.	Heat,Hot Temperatures,Temperature, Hot,Temperatures, Hot
D006863	Hydrogen-Ion Concentration	The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH	pH,Concentration, Hydrogen-Ion,Concentrations, Hydrogen-Ion,Hydrogen Ion Concentration,Hydrogen-Ion Concentrations
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia