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Heat-induced G1 arrest is dependent on p53 function but not on RB dephosphorylation. 1999

M Miyakoda, and K Nakahata, and K Suzuki, and S Kodama, and M Watanabe
School of Pharmaceutical Sciences, Nagasaki University, Nagasaki, 852-8521, Japan.

Normal human cells were heat shocked at 43 degrees C for 2 hr and recovered at 37 degrees C. The levels of p53 and p21 reached a maximum approximately 2 and 6 hr after the heat shock, respectively, and these levels were higher than the control level even at 24 hr. The fraction of S phase cells decreased significantly 8 hr after heat shock, and gradually thereafter. Heat-induced G1 arrest was not found in NCI-H1299 and HeLa cells, which are deficient in p53 function, indicating that p53 function is essential for G1 arrest after heat shock. We found little or no change in phosphorylation of retinoblastoma (RB) protein until 12 hr after heat shock, and a significant change at about 24 hr. No change in phosphorylation of RB at serine-780 and serine-795 occurred within 12 hr after heat shock. These results suggest that heat shock induces G1 arrest mediated by p53, but that G1 arrest within 12 hr after heat shock does not require RB dephosphorylation.

UI MeSH Term Description Entries
D010766 Phosphorylation The introduction of a phosphoryl group into a compound through the formation of an ester bond between the compound and a phosphorus moiety. Phosphorylations
D002460 Cell Line Established cell cultures that have the potential to propagate indefinitely. Cell Lines,Line, Cell,Lines, Cell
D006358 Hot Temperature Presence of warmth or heat or a temperature notably higher than an accustomed norm. Heat,Hot Temperatures,Temperature, Hot,Temperatures, Hot
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D016159 Tumor Suppressor Protein p53 Nuclear phosphoprotein encoded by the p53 gene (GENES, P53) whose normal function is to control CELL PROLIFERATION and APOPTOSIS. A mutant or absent p53 protein has been found in LEUKEMIA; OSTEOSARCOMA; LUNG CANCER; and COLORECTAL CANCER. p53 Tumor Suppressor Protein,Cellular Tumor Antigen p53,Oncoprotein p53,TP53 Protein,TRP53 Protein,p53 Antigen,pp53 Phosphoprotein,Phosphoprotein, pp53
D016160 Retinoblastoma Protein Product of the retinoblastoma tumor suppressor gene. It is a nuclear phosphoprotein hypothesized to normally act as an inhibitor of cell proliferation. Rb protein is absent in retinoblastoma cell lines. It also has been shown to form complexes with the adenovirus E1A protein, the SV40 T antigen, and the human papilloma virus E7 protein. Rb Protein,Retinoblastoma Nuclear Phosphoprotein p105-Rb,p105-Rb Protein,Rb Gene Product,Rb1 Gene Product,Retinoblastoma Nuclear Phosphoprotein p105 Rb,p105 Rb Protein
D016193 G1 Phase The period of the CELL CYCLE preceding DNA REPLICATION in S PHASE. Subphases of G1 include "competence" (to respond to growth factors), G1a (entry into G1), G1b (progression), and G1c (assembly). Progression through the G1 subphases is effected by limiting growth factors, nutrients, or inhibitors. First Gap Phase,G1a Phase,G1b Phase,Gap Phase 1,First Gap Phases,G1 Phases,G1a Phases,G1b Phases,Gap Phase, First,Gap Phases, First,Phase 1, Gap,Phase, First Gap,Phase, G1,Phase, G1a,Phase, G1b,Phases, First Gap,Phases, G1,Phases, G1a,Phases, G1b
D016196 S Phase Phase of the CELL CYCLE following G1 and preceding G2 when the entire DNA content of the nucleus is replicated. It is achieved by bidirectional replication at multiple sites along each chromosome. S Period,Period, S,Periods, S,Phase, S,Phases, S,S Periods,S Phases
D019925 Cyclin A A cyclin subtype that has specificity for CDC2 PROTEIN KINASE and CYCLIN-DEPENDENT KINASE 2. It plays a role in progression of the CELL CYCLE through G1/S and G2/M phase transitions. Adenovirus E1A-Associated Protein p60,E1A p60,Type A Cyclin,Type A Cyclins,Adenovirus E1A Associated Protein p60,Cyclin, Type A,Cyclins, Type A
D020741 rho GTP-Binding Proteins A large family of MONOMERIC GTP-BINDING PROTEINS that are involved in regulation of actin organization, gene expression and cell cycle progression. This enzyme was formerly listed as EC 3.6.1.47. rho G-Proteins,rho GTPase,rho GTPases,rho Small GTP-Binding Proteins,P21 (rho)Protein,rho GTP-Binding Protein,rho Protein P21,G-Proteins, rho,GTP-Binding Protein, rho,GTP-Binding Proteins, rho,GTPase, rho,GTPases, rho,P21, rho Protein,rho G Proteins,rho GTP Binding Protein,rho GTP Binding Proteins,rho Small GTP Binding Proteins

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