BIOMAT Database Logo BIOMAT Database Logo

Glucagon-like peptide-2 increases sucrase-isomaltase but not caudal-related homeobox protein-2 gene expression. 2000

P A Kitchen, and A J Fitzgerald, and R A Goodlad, and N F Barley, and M A Ghatei, and S Legon, and S R Bloom, and A Price, and J R Walters, and A Forbes
St Mark's Hospital, Harrow HA1 3UJ, United Kingdom. p.kitchen@ic.ac.uk

To determine the effect of glucagon-like peptide-2 (GLP-2) on sucrase-isomaltase and caudal-related homeobox protein-2 (Cdx-2) gene expression, male Wistar rats were divided into total parenteral nutrition (TPN)-fed and GLP-2-treated, TPN-fed groups. TPN was given via a jugular line, inserted under anesthesia, for 7 days. The treatment group received 40 microg/day of GLP-2 intravenously with the TPN diet. The small intestine and colon were weighed and measured. Tissue was obtained from the jejunum, terminal ileum, and midcolon. RNA analysis, morphometry, and microdissection were performed. The weight of the small intestine of GLP-2-treated rats was greater than that of TPN-fed rats (P < 0.001). GLP-2 increased the mean metaphase arrests/crypt in both the jejunum and ileum (P < 0.001). Ileal expression of sucrase-isomaltase was increased by 1. 6-fold (P < 0.05). Jejunal expression was increased by a similar amount, although not significantly (P = 0.08). There was no change in Cdx-2 gene expression. Thus GLP-2 can maintain small intestinal morphology and function, but effects on gene expression are not mediated by gross changes in the level of the mRNA for the homeobox protein Cdx-2.

UI MeSH Term Description Entries
D007082 Ileum The distal and narrowest portion of the SMALL INTESTINE, between the JEJUNUM and the ILEOCECAL VALVE of the LARGE INTESTINE.
D007583 Jejunum The middle portion of the SMALL INTESTINE, between DUODENUM and ILEUM. It represents about 2/5 of the remaining portion of the small intestine below duodenum. Jejunums
D008297 Male Males
D010289 Parenteral Nutrition, Total The delivery of nutrients for assimilation and utilization by a patient whose sole source of nutrients is via solutions administered intravenously, subcutaneously, or by some other non-alimentary route. The basic components of TPN solutions are protein hydrolysates or free amino acid mixtures, monosaccharides, and electrolytes. Components are selected for their ability to reverse catabolism, promote anabolism, and build structural proteins. Hyperalimentation, Parenteral,Intravenous Hyperalimentation,Nutrition, Total Parenteral,Parenteral Hyperalimentation,Total Parenteral Nutrition,Hyperalimentation, Intravenous
D010455 Peptides Members of the class of compounds composed of AMINO ACIDS joined together by peptide bonds between adjacent amino acids into linear, branched or cyclical structures. OLIGOPEPTIDES are composed of approximately 2-12 amino acids. Polypeptides are composed of approximately 13 or more amino acids. PROTEINS are considered to be larger versions of peptides that can form into complex structures such as ENZYMES and RECEPTORS. Peptide,Polypeptide,Polypeptides
D003106 Colon The segment of LARGE INTESTINE between the CECUM and the RECTUM. It includes the ASCENDING COLON; the TRANSVERSE COLON; the DESCENDING COLON; and the SIGMOID COLON. Appendix Epiploica,Taenia Coli,Omental Appendices,Omental Appendix,Appendices, Omental,Appendix, Omental
D004763 Glucagon-Like Peptides Peptides derived from proglucagon which is also the precursor of pancreatic GLUCAGON. Despite expression of proglucagon in multiple tissues, the major production site of glucagon-like peptides (GLPs) is the INTESTINAL L CELLS. GLPs include glucagon-like peptide 1, glucagon-like peptide 2, and the various truncated forms. Enteroglucagon,Enteroglucagons,Glucagon-Like Peptide,Glucagon-Like Polypeptide,Gut Glucagon,Glucagon-Like Polypeptides,Glucagon Like Peptide,Glucagon Like Peptides,Glucagon Like Polypeptide,Glucagon Like Polypeptides,Glucagon, Gut,Peptide, Glucagon-Like,Polypeptide, Glucagon-Like
D000071616 CDX2 Transcription Factor An antennapedia-like homeodomain transcription factor that regulates the expression of multiple genes in the INTESTINAL MUCOSA. It plays a critical role in many processes from early differentiation to maintenance of the intestinal epithelial lining of both the small and large intestine. CDX2 Protein,Caudal Type Homeo Box 2 Protein,Caudal Type Homeo Box Transcription Factor 2,Caudal Type Homeobox Transcription Factor 2,Cdx-2 Protein,Cdx-2-3 Protein,Cdx-3 Protein,Homeobox Protein CDX-2,Homeobox Protein CDX2,Transcription Factor CDX2,CDX-2, Homeobox Protein,CDX2, Homeobox Protein,CDX2, Transcription Factor,Cdx 2 3 Protein,Cdx 2 Protein,Cdx 3 Protein,Homeobox Protein CDX 2
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D013394 Sucrase-Isomaltase Complex An enzyme complex found in the brush border membranes of the small intestine. It is believed to be an enzyme complex with different catalytic sites. Its absence is manifested by an inherited disease called sucrase-isomaltase deficiency. Sucrase Isomaltase Complex,Complex, Sucrase Isomaltase,Complex, Sucrase-Isomaltase,Isomaltase Complex, Sucrase

Related Publications

P A Kitchen, and A J Fitzgerald, and R A Goodlad, and N F Barley, and M A Ghatei, and S Legon, and S R Bloom, and A Price, and J R Walters, and A Forbes
Glucagon-like peptide 2 enhances maltase-glucoamylase and sucrase-isomaltase gene expression and activity in parenterally fed premature neonatal piglets.
October 2002, Pediatric research,
P A Kitchen, and A J Fitzgerald, and R A Goodlad, and N F Barley, and M A Ghatei, and S Legon, and S R Bloom, and A Price, and J R Walters, and A Forbes
Lactase and sucrase-isomaltase gene expression during Caco-2 cell differentiation.
June 1995, The Biochemical journal,
P A Kitchen, and A J Fitzgerald, and R A Goodlad, and N F Barley, and M A Ghatei, and S Legon, and S R Bloom, and A Price, and J R Walters, and A Forbes
Glucagon-like peptide-1 but not glucagon-like peptide-2 stimulates insulin release from isolated rat pancreatic islets.
September 1985, Diabetologia,
P A Kitchen, and A J Fitzgerald, and R A Goodlad, and N F Barley, and M A Ghatei, and S Legon, and S R Bloom, and A Price, and J R Walters, and A Forbes
Sucrase-isomaltase gene expression in Barrett's esophagus and adenocarcinoma.
September 1993, Gastroenterology,
P A Kitchen, and A J Fitzgerald, and R A Goodlad, and N F Barley, and M A Ghatei, and S Legon, and S R Bloom, and A Price, and J R Walters, and A Forbes
The caudal-related homeodomain protein Cdx-2/3 regulates glucagon gene expression in islet cells.
November 1996, The Journal of biological chemistry,
P A Kitchen, and A J Fitzgerald, and R A Goodlad, and N F Barley, and M A Ghatei, and S Legon, and S R Bloom, and A Price, and J R Walters, and A Forbes
Glucagon-like peptide-2 inhibits antral emptying in man, but is not as potent as glucagon-like peptide-1.
April 2004, Scandinavian journal of gastroenterology,
P A Kitchen, and A J Fitzgerald, and R A Goodlad, and N F Barley, and M A Ghatei, and S Legon, and S R Bloom, and A Price, and J R Walters, and A Forbes
Pou homeodomain protein OCT1 is implicated in the expression of the caudal-related homeobox gene Cdx-2.
May 2001, The Journal of biological chemistry,
P A Kitchen, and A J Fitzgerald, and R A Goodlad, and N F Barley, and M A Ghatei, and S Legon, and S R Bloom, and A Price, and J R Walters, and A Forbes
Dietary regulation of sucrase-isomaltase gene expression in rat jejunum.
February 1995, Biochimica et biophysica acta,
P A Kitchen, and A J Fitzgerald, and R A Goodlad, and N F Barley, and M A Ghatei, and S Legon, and S R Bloom, and A Price, and J R Walters, and A Forbes
Sucrase-isomaltase deficiency in 2 siblings.
December 1972, Journal of the Irish Medical Association,
P A Kitchen, and A J Fitzgerald, and R A Goodlad, and N F Barley, and M A Ghatei, and S Legon, and S R Bloom, and A Price, and J R Walters, and A Forbes
Molecular cloning and characterization of a rat intestinal sucrase-isomaltase cDNA. Regulation of sucrase-isomaltase gene expression by sucrose feeding.
September 1990, Biochimica et biophysica acta,
Copied contents to your clipboard!