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The active thrombin is formed in the blood stream when the blood coagulation system is activated. It attacks fibrinogen, splits off two fibrinopeptides A and fibrinogen is transformed into des-AA fibrin which is able to polymerize spontaneously forming protofibrils. At high thrombin concentration the enzyme splits off two fibrinopeptides B and des-AA fibrin units are transformed into des-AABB fibrin. These two forms of fibrin are widely used in the biological experiments. However des-AA fibrin is obtained usually from fibrinogen using the snake poisons (such as reptilase). Des-AA fibrin was obtained also by physiological enzyme thrombin, but that des-AA fibrin samples had the contamination of des-AABB fibrin. At the present paper we have described the method of the des-AA fibrin preparation by thrombin without any contamination of des-AABB fibrin.
E V Lougovskoi, and
G K Gogolinskaya
November 1978,
Thrombosis research,
E V Lougovskoi, and
G K Gogolinskaya
June 1986,
Thrombosis and haemostasis,
E V Lougovskoi, and
G K Gogolinskaya
January 1985,
Scandinavian journal of clinical and laboratory investigation. Supplementum,
E V Lougovskoi, and
G K Gogolinskaya
November 1978,
Thrombosis research,
E V Lougovskoi, and
G K Gogolinskaya
January 1979,
Thrombosis research,
E V Lougovskoi, and
G K Gogolinskaya
April 1982,
Biochemical and biophysical research communications,
E V Lougovskoi, and
G K Gogolinskaya
January 1980,
Thrombosis research,
E V Lougovskoi, and
G K Gogolinskaya
January 1979,
Thrombosis research,
E V Lougovskoi, and
G K Gogolinskaya
November 1973,
Biochimica et biophysica acta,
E V Lougovskoi, and
G K Gogolinskaya
October 1990,
The Journal of biological chemistry,
