Biomaterial Database

Chloride transport in isolated frog (Rana temporaria) skin: changes in short-circuit currently, potential, resistance, and chloride flux elicited by furosemide. 1976

C J Lote

In a large number of isolated frog skins, with potential differences of from 20--92 mV (mean, 55.3 +/- 3.6 S.E.M.), the chloride influx was found to be slightly greater than chloride efflux under short-circuit conditions, but the difference was not statistically significant. However, if skins of low potential (less than 50 mV) were selected, chloride influx was significantly higher than chloride efflux (P less than 0.05). In this low potential group furosemide, (10(-3)M, applied to the solution bathing the mucosal surface) was found to produce a) a small increase in short-circuit current, which was generally apparent within 1 min, maximal in 5 min; and thereafter declined towards the control value; b) a marked increase in potential difference, apparent within 1 min and sustained for at least 30 min; c) a large and sustained increase in the calculated d.c. resistance of the skin and d) a decrease in the influx of chloride, such that influx and efflux were equalized.

UI	MeSH Term	Description	Entries
D011896	Rana temporaria	A species of the family Ranidae occurring in a wide variety of habitats from within the Arctic Circle to South Africa, Australia, etc.	European Common Frog,Frog, Common European,Common European Frog,Common Frog, European,European Frog, Common,Frog, European Common
D002712	Chlorides	Inorganic compounds derived from hydrochloric acid that contain the Cl- ion.	Chloride,Chloride Ion Level,Ion Level, Chloride,Level, Chloride Ion
D005665	Furosemide	A benzoic-sulfonamide-furan. It is a diuretic with fast onset and short duration that is used for EDEMA and chronic RENAL INSUFFICIENCY.	Frusemide,Fursemide,Errolon,Frusemid,Furanthril,Furantral,Furosemide Monohydrochloride,Furosemide Monosodium Salt,Fusid,Lasix
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D001693	Biological Transport, Active	The movement of materials across cell membranes and epithelial layers against an electrochemical gradient, requiring the expenditure of metabolic energy.	Active Transport,Uphill Transport,Active Biological Transport,Biologic Transport, Active,Transport, Active Biological,Active Biologic Transport,Transport, Active,Transport, Active Biologic,Transport, Uphill
D012867	Skin	The outer covering of the body that protects it from the environment. It is composed of the DERMIS and the EPIDERMIS.