Kluyveromyces marxianus exhibits an ancestral Saccharomyces cerevisiae genome organization downstream of ADH2. 2000

J M Ladrière, and I Georis, and M Guérineau, and J Vandenhaute
Unité de Recherche en Biologie Moléculaire, Laboratoire de Génétique Moléculaire, Facultés Universitaires Notre-Dame de la Paix, Namur, Belgium. jean-marc.ladriere@dsm-group.com

In Saccharomyces cerevisiae, the alcohol dehydrogenase genes ADH1 and ADH5 are part of a duplicated block of genome, thought to originate from a genome-wide duplication posterior to the divergence from the Kluyveromyces lineage. We report here the characterization of Kluyveromyces marxianus ADH2 and the five genes found in its immediate downstream region, MRPS9, YOL087C, RPB5, RIB7 and SPP381. The order of these six genes reflects the structure of the ancestral S. cerevisiae genome before the duplication that formed the blocks including ADH1 on chromosome XV and ADH5 on chromosome II, indicating these ADH genes share a direct ancestor. On the one hand, the two genes found immediately downstream of KmADH2 are located, for the first, downstream ADH5 and, for the second, downstream ADH1 in S. cerevisiae. On the other hand, the order of the paralogs included in the blocks of ADH1 and ADH5 in S. cerevisiae suggests that two of them have been inverted within one block after its formation, and that inversion is confirmed by the gene order observed in K. marxianus.

UI MeSH Term Description Entries
D007527 Isoenzymes Structurally related forms of an enzyme. Each isoenzyme has the same mechanism and classification, but differs in its chemical, physical, or immunological characteristics. Alloenzyme,Allozyme,Isoenzyme,Isozyme,Isozymes,Alloenzymes,Allozymes
D007716 Kluyveromyces An ascomycetous yeast of the fungal family Saccharomycetaceae, order SACCHAROMYCETALES. Kluyveromyce
D008969 Molecular Sequence Data Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories. Sequence Data, Molecular,Molecular Sequencing Data,Data, Molecular Sequence,Data, Molecular Sequencing,Sequencing Data, Molecular
D003001 Cloning, Molecular The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells. Molecular Cloning
D004271 DNA, Fungal Deoxyribonucleic acid that makes up the genetic material of fungi. Fungal DNA
D005656 Fungal Proteins Proteins found in any species of fungus. Fungal Gene Products,Fungal Gene Proteins,Fungal Peptides,Gene Products, Fungal,Yeast Proteins,Gene Proteins, Fungal,Peptides, Fungal,Proteins, Fungal
D000091243 Ribosomal Protein S9 It is a protein that encodes a RIBOSOMAL PROTEIN which is a component of the 40S subunit. It belongs to the S4P family of ribosomal proteins. S9 Ribosomal Protein,Protein S9, Ribosomal,Protein, S9 Ribosomal,S9, Ribosomal Protein
D000426 Alcohol Dehydrogenase A zinc-containing enzyme which oxidizes primary and secondary alcohols or hemiacetals in the presence of NAD. In alcoholic fermentation, it catalyzes the final step of reducing an aldehyde to an alcohol in the presence of NADH and hydrogen. Alcohol Dehydrogenase (NAD+),Alcohol Dehydrogenase I,Alcohol Dehydrogenase II,Alcohol-NAD+ Oxidoreductase,Yeast Alcohol Dehydrogenase,Alcohol Dehydrogenase, Yeast,Alcohol NAD+ Oxidoreductase,Dehydrogenase, Alcohol,Dehydrogenase, Yeast Alcohol,Oxidoreductase, Alcohol-NAD+
D000595 Amino Acid Sequence The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION. Protein Structure, Primary,Amino Acid Sequences,Sequence, Amino Acid,Sequences, Amino Acid,Primary Protein Structure,Primary Protein Structures,Protein Structures, Primary,Structure, Primary Protein,Structures, Primary Protein
D012441 Saccharomyces cerevisiae A species of the genus SACCHAROMYCES, family Saccharomycetaceae, order Saccharomycetales, known as "baker's" or "brewer's" yeast. The dried form is used as a dietary supplement. Baker's Yeast,Brewer's Yeast,Candida robusta,S. cerevisiae,Saccharomyces capensis,Saccharomyces italicus,Saccharomyces oviformis,Saccharomyces uvarum var. melibiosus,Yeast, Baker's,Yeast, Brewer's,Baker Yeast,S cerevisiae,Baker's Yeasts,Yeast, Baker

Related Publications

J M Ladrière, and I Georis, and M Guérineau, and J Vandenhaute
December 2000, Current genetics,
J M Ladrière, and I Georis, and M Guérineau, and J Vandenhaute
October 2022, World journal of microbiology & biotechnology,
J M Ladrière, and I Georis, and M Guérineau, and J Vandenhaute
January 2015, Mikrobiologiia,
J M Ladrière, and I Georis, and M Guérineau, and J Vandenhaute
March 2013, Applied microbiology and biotechnology,
J M Ladrière, and I Georis, and M Guérineau, and J Vandenhaute
January 2022, Preparative biochemistry & biotechnology,
J M Ladrière, and I Georis, and M Guérineau, and J Vandenhaute
November 2019, Environmental microbiology,
J M Ladrière, and I Georis, and M Guérineau, and J Vandenhaute
September 2012, Journal of bioscience and bioengineering,
J M Ladrière, and I Georis, and M Guérineau, and J Vandenhaute
August 2014, World journal of microbiology & biotechnology,
J M Ladrière, and I Georis, and M Guérineau, and J Vandenhaute
February 2023, Journal of applied microbiology,
J M Ladrière, and I Georis, and M Guérineau, and J Vandenhaute
May 2013, World journal of microbiology & biotechnology,
Copied contents to your clipboard!