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We describe a "sandwich-type" enzyme immunoassay for insulin in serum, in which antibody Fab'-beta-D-galactosidase conjugate and an antibody-immobilized silicone rubber solid-phase are used. The interference by serum factors with the solid-phase enzyme immunoassay can now be removed by using a buffer containing gelatin. Serum samples of 50 microL can be analyzed by the enzyme immunoassay, which is as sensitive as radioimmunoassay for human insulin. Our results correlate well with those for radioimmunoassay (r = 0.97, slope = 0.92, y-intercept = 4.6 milli-int. units /L for 181 samples). Between-assay and within-assay coefficients of variation are less than 15% over the useful ranges of the assay (5--160 milli-int. units/L).
K Kato, and
Y Umeda, and
F Suzuki, and
D Hayashi, and
A Kosaka
April 1989,
Journal of immunological methods,
K Kato, and
Y Umeda, and
F Suzuki, and
D Hayashi, and
A Kosaka
November 1977,
Clinical chemistry,
K Kato, and
Y Umeda, and
F Suzuki, and
D Hayashi, and
A Kosaka
August 1979,
Clinical chemistry,
K Kato, and
Y Umeda, and
F Suzuki, and
D Hayashi, and
A Kosaka
January 1991,
Journal of immunological methods,
K Kato, and
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November 1983,
Clinical chemistry,
K Kato, and
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A Kosaka
May 1981,
Clinica chimica acta; international journal of clinical chemistry,
K Kato, and
Y Umeda, and
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January 1986,
Clinical chemistry,
K Kato, and
Y Umeda, and
F Suzuki, and
D Hayashi, and
A Kosaka
January 1986,
Zeitschrift fur medizinische Laboratoriumsdiagnostik,
K Kato, and
Y Umeda, and
F Suzuki, and
D Hayashi, and
A Kosaka
November 1985,
Journal of immunological methods,
K Kato, and
Y Umeda, and
F Suzuki, and
D Hayashi, and
A Kosaka
January 1979,
Clinical chemistry,
