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A solid-phase enzyme immunoassay for the measurement of anti-insulin antibodies in the sera of patients with diabetes mellitus was developed. Porcine insulin conjugated with bovine serum albumin was used for coating microtiter plates. This assay was as sensitive as the conventional radioimmunoassay. Anti-insulin antibody titers measured by the enzyme immunoassay and conventional radioimmunoassay correlated well and the correlation coefficient was 0.920, which was statistically significant (P less than 0.001). The enzyme immunoassay detected anti-insulin antibody in 23 out of 35 sera of patients with insulin-dependent diabetes mellitus. The present enzyme immunoassay does not require radioactive materials, is less expensive and is concluded to be practically useful.
N Kobayashi, and
E Terada, and
A Ghoda, and
K Kitagaki, and
H Okudaira, and
T Ogita, and
K Shimabukuro, and
T Miyamoto
April 1989,
Journal of immunological methods,
N Kobayashi, and
E Terada, and
A Ghoda, and
K Kitagaki, and
H Okudaira, and
T Ogita, and
K Shimabukuro, and
T Miyamoto
July 1979,
Clinical chemistry,
N Kobayashi, and
E Terada, and
A Ghoda, and
K Kitagaki, and
H Okudaira, and
T Ogita, and
K Shimabukuro, and
T Miyamoto
July 1984,
Nihon Naibunpi Gakkai zasshi,
N Kobayashi, and
E Terada, and
A Ghoda, and
K Kitagaki, and
H Okudaira, and
T Ogita, and
K Shimabukuro, and
T Miyamoto
November 1977,
Applied and environmental microbiology,
N Kobayashi, and
E Terada, and
A Ghoda, and
K Kitagaki, and
H Okudaira, and
T Ogita, and
K Shimabukuro, and
T Miyamoto
May 1987,
Journal of virological methods,
N Kobayashi, and
E Terada, and
A Ghoda, and
K Kitagaki, and
H Okudaira, and
T Ogita, and
K Shimabukuro, and
T Miyamoto
November 1977,
Clinical chemistry,
N Kobayashi, and
E Terada, and
A Ghoda, and
K Kitagaki, and
H Okudaira, and
T Ogita, and
K Shimabukuro, and
T Miyamoto
January 1980,
Journal of immunological methods,
N Kobayashi, and
E Terada, and
A Ghoda, and
K Kitagaki, and
H Okudaira, and
T Ogita, and
K Shimabukuro, and
T Miyamoto
June 1977,
Immunochemistry,
N Kobayashi, and
E Terada, and
A Ghoda, and
K Kitagaki, and
H Okudaira, and
T Ogita, and
K Shimabukuro, and
T Miyamoto
December 2007,
Klinicheskaia laboratornaia diagnostika,
N Kobayashi, and
E Terada, and
A Ghoda, and
K Kitagaki, and
H Okudaira, and
T Ogita, and
K Shimabukuro, and
T Miyamoto
August 1979,
Clinical chemistry,
