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Activation of platelet-derived growth factor-beta (PDGF-beta) receptors in the nucleus of the solitary tract (nTS) modulates the late phase of the acute hypoxic ventilatory response (HVR) in the rat. We hypothesized that temporal changes in PDGF-beta receptor expression could underlie the ventilatory acclimatization to hypoxia (VAH). Normoxic ventilation was examined in adult Sprague-Dawley rats chronically exposed to 10% O(2), and at 0, 1, 2, 7, and 14 days, Northern and Western blots of the dorsocaudal brain stem were performed for assessment of PDGF-beta receptor expression. Although no significant changes in PDGF-beta receptor mRNA occurred over time, marked attenuation of PDGF-beta receptor protein became apparent after day 7 of hypoxic exposure. Such changes were significantly correlated with concomitant increases in normoxic ventilation, i.e., with VAH (r: -0.56, P < 0.005). In addition, long-term administration of PDGF-BB in the nTS via osmotic pumps loaded with either PDGF-BB (n = 8) or vehicle (Veh; n = 8) showed that although no significant changes in the magnitude of acute HVR occurred in Veh over time, the typical attenuation of HVR by PDGF-BB decreased over time. Furthermore, PDGF-BB microinjections did not attenuate HVR in acclimatized rats at 7 and 14 days of hypoxia (n = 10). We conclude that decreased expression of PDGF-beta receptors in the dorsocaudal brain stem correlates with the magnitude of VAH. We speculate that the decreased expression of PDGF-beta receptors is mediated via internalization and degradation of the receptor rather than by transcriptional regulation.
O A Alea, and
M A Czapla, and
J A Lasky, and
N Simakajornboon, and
E Gozal, and
D Gozal
August 2001,
Pediatric research,
O A Alea, and
M A Czapla, and
J A Lasky, and
N Simakajornboon, and
E Gozal, and
D Gozal
September 2000,
Respiration physiology,
O A Alea, and
M A Czapla, and
J A Lasky, and
N Simakajornboon, and
E Gozal, and
D Gozal
December 2008,
Respiratory physiology & neurobiology,
O A Alea, and
M A Czapla, and
J A Lasky, and
N Simakajornboon, and
E Gozal, and
D Gozal
September 1996,
Brain research. Molecular brain research,
O A Alea, and
M A Czapla, and
J A Lasky, and
N Simakajornboon, and
E Gozal, and
D Gozal
January 2017,
Respiratory physiology & neurobiology,
O A Alea, and
M A Czapla, and
J A Lasky, and
N Simakajornboon, and
E Gozal, and
D Gozal
June 1994,
The Journal of physiology,
O A Alea, and
M A Czapla, and
J A Lasky, and
N Simakajornboon, and
E Gozal, and
D Gozal
April 2014,
The Journal of physiology,
O A Alea, and
M A Czapla, and
J A Lasky, and
N Simakajornboon, and
E Gozal, and
D Gozal
January 1994,
Advances in experimental medicine and biology,
O A Alea, and
M A Czapla, and
J A Lasky, and
N Simakajornboon, and
E Gozal, and
D Gozal
April 1993,
Respiration physiology,
O A Alea, and
M A Czapla, and
J A Lasky, and
N Simakajornboon, and
E Gozal, and
D Gozal
January 2016,
PloS one,
