-transparent.png){kind=logo}
In adrenal cortex, ACTH regulation of steroidogenesis depends on PKA-dependent serine/threonine phosphorylation and also on the activity of protein tyrosine phosphatases (PTPs). In addition, ACTH increases total PTPs involving at least three soluble PTPs (50, 82 and 115 kDa). Serine/threonine phosphorylation of these enzymes themselves could be a regulatory mechanism of their activity since the increase of total PTP activity is dependent on PKA-activation. In this report we analyzed the effect of in vitro phospho-dephosphorylation processes on the activity displayed by the ACTH-activated PTP of 115 kDa. Using an in-gel PTP assay we demonstrate that dephosphorylation catalyzed by potato acid phosphatase (PAP) reduces the activity of the 115 kDa PTP present in ZF from ACTH-treated animals and PKA-mediated phosphorylation reverses this effect.
C Paz, and
F Cornejo Maciel, and
C Poderoso, and
A Gorostizaga, and
E J Podestá
August 1994,
The EMBO journal,
C Paz, and
F Cornejo Maciel, and
C Poderoso, and
A Gorostizaga, and
E J Podestá
May 2001,
Molecular and cellular biochemistry,
C Paz, and
F Cornejo Maciel, and
C Poderoso, and
A Gorostizaga, and
E J Podestá
August 1994,
Molecular and cellular biology,
C Paz, and
F Cornejo Maciel, and
C Poderoso, and
A Gorostizaga, and
E J Podestá
February 2023,
The Journal of clinical investigation,
C Paz, and
F Cornejo Maciel, and
C Poderoso, and
A Gorostizaga, and
E J Podestá
November 2016,
The Journal of clinical investigation,
C Paz, and
F Cornejo Maciel, and
C Poderoso, and
A Gorostizaga, and
E J Podestá
May 2016,
The Journal of clinical investigation,
C Paz, and
F Cornejo Maciel, and
C Poderoso, and
A Gorostizaga, and
E J Podestá
January 2024,
Methods in molecular biology (Clifton, N.J.),
C Paz, and
F Cornejo Maciel, and
C Poderoso, and
A Gorostizaga, and
E J Podestá
June 2013,
Molecular and cellular biology,
C Paz, and
F Cornejo Maciel, and
C Poderoso, and
A Gorostizaga, and
E J Podestá
January 1994,
FEBS letters,
C Paz, and
F Cornejo Maciel, and
C Poderoso, and
A Gorostizaga, and
E J Podestá
June 2006,
American journal of physiology. Cell physiology,
