Biomaterial Database

Enzyme deletions and essential fatty acid metabolism in cultured cells. 1975

L Mathers, and M J Bailey

Conversion of 14C-labeled linoleic acid to dihomo-gamma-linolenic and arachidonic acids, precursors of the PG1 and PG2 series of prostaglandins, was examined in a number of mammalian cell lines in tissue culture. Diploid cells converted linoleate to arachidonate in good yield. Heteroploid or transformed cells did not desaturate linoleic acid but readily converted dihomo-gamma-[14C]linolenate to arachidonate. These observations demonstrate that separate delta6 and delta5 desaturases are involved in the conversion of linoleic to arachidonic acid in mammals. The results indicate that loss or modification of the delta6 desaturase system is a frequent occurrence in transformed cells.

UI	MeSH Term	Description	Entries
D008041	Linoleic Acids	Eighteen-carbon essential fatty acids that contain two double bonds.	Acids, Linoleic
D008042	Linolenic Acids	Eighteen-carbon essential fatty acids that contain three double bonds.
D009154	Mutation	Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.	Mutations
D002460	Cell Line	Established cell cultures that have the potential to propagate indefinitely.	Cell Lines,Line, Cell,Lines, Cell
D002471	Cell Transformation, Neoplastic	Cell changes manifested by escape from control mechanisms, increased growth potential, alterations in the cell surface, karyotypic abnormalities, morphological and biochemical deviations from the norm, and other attributes conferring the ability to invade, metastasize, and kill.	Neoplastic Transformation, Cell,Neoplastic Cell Transformation,Transformation, Neoplastic Cell,Tumorigenic Transformation,Cell Neoplastic Transformation,Cell Neoplastic Transformations,Cell Transformations, Neoplastic,Neoplastic Cell Transformations,Neoplastic Transformations, Cell,Transformation, Cell Neoplastic,Transformation, Tumorigenic,Transformations, Cell Neoplastic,Transformations, Neoplastic Cell,Transformations, Tumorigenic,Tumorigenic Transformations
D004171	Diploidy	The chromosomal constitution of cells, in which each type of CHROMOSOME is represented twice. Symbol: 2N or 2X.	Diploid,Diploid Cell,Cell, Diploid,Cells, Diploid,Diploid Cells,Diploidies,Diploids
D005228	Fatty Acids, Essential	Long chain organic acid molecules that must be obtained from the diet. Examples are LINOLEIC ACIDS and LINOLENIC ACIDS.	Acids, Essential Fatty,Essential Fatty Acids
D001095	Arachidonic Acids		Eicosatetraenoic Acids,Acids, Arachidonic,Acids, Eicosatetraenoic
D044943	Fatty Acid Desaturases	A family of enzymes that catalyze the stereoselective, regioselective, or chemoselective syn-dehydrogenation reactions. They function by a mechanism that is linked directly to reduction of molecular OXYGEN.	Acyl CoA Desaturase,Enoyl CoA Reductase,Fatty Acid Desaturase,Fatty Acid Desaturating Enzymes,Acyl CoA Desaturases,Enoyl CoA Reductases,Acid Desaturase, Fatty,CoA Desaturase, Acyl,CoA Reductase, Enoyl,Desaturase, Acyl CoA,Desaturase, Fatty Acid,Desaturases, Fatty Acid,Reductase, Enoyl CoA,Reductases, Enoyl CoA