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Human gamma/delta T-cell lines derived from airway biopsies. 2001

A V Wisnewski, and H Cain, and N Magoski, and H Wang, and C T Holm, and C A Redlich
Pulmonary and Critical Care Section, Yale School of Medicine, New Haven, Connecticut 06520, USA. adam.wisnewski@yale.edu

gamma/delta T cells have been postulated to play an important role in the immune response at epithelial boundaries, but have not been well described in human lung tissue. We have identified and characterized gamma/delta T-cell lines from human airway biopsies and compared them with T-cell lines from paired peripheral blood samples. Airway-derived T-cell lines stimulated with tetanus toxoid (TT) contained a greater proportion of gamma/delta T cells compared with T-cell lines stimulated with mitogens, other antigens, or without antigen. TT-stimulated airway T cells expressed different T-cell receptors (TCRs) than did blood- derived T cells, and used predominantly variable region (V)gamma I family genes rather than V gamma II family genes. Airway-derived gamma/delta T cells produced high levels of interferon-gamma and were associated with T helper 1--like cytokine profiles. This study describes the presence and antigen-dependent proliferation of gamma/delta T cells from human airway tissue, and demonstrates differences in lung-derived gamma/delta TCRs compared with gamma/delta T cells derived from peripheral blood. The data suggest that gamma/delta T cells may be functionally enriched in human airways relative to peripheral blood.

UI MeSH Term Description Entries
D008213 Lymphocyte Activation Morphologic alteration of small B LYMPHOCYTES or T LYMPHOCYTES in culture into large blast-like cells able to synthesize DNA and RNA and to divide mitotically. It is induced by INTERLEUKINS; MITOGENS such as PHYTOHEMAGGLUTININS, and by specific ANTIGENS. It may also occur in vivo as in GRAFT REJECTION. Blast Transformation,Blastogenesis,Lymphoblast Transformation,Lymphocyte Stimulation,Lymphocyte Transformation,Transformation, Blast,Transformation, Lymphoblast,Transformation, Lymphocyte,Activation, Lymphocyte,Stimulation, Lymphocyte
D002460 Cell Line Established cell cultures that have the potential to propagate indefinitely. Cell Lines,Line, Cell,Lines, Cell
D005434 Flow Cytometry Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake. Cytofluorometry, Flow,Cytometry, Flow,Flow Microfluorimetry,Fluorescence-Activated Cell Sorting,Microfluorometry, Flow,Cell Sorting, Fluorescence-Activated,Cell Sortings, Fluorescence-Activated,Cytofluorometries, Flow,Cytometries, Flow,Flow Cytofluorometries,Flow Cytofluorometry,Flow Cytometries,Flow Microfluorometries,Flow Microfluorometry,Fluorescence Activated Cell Sorting,Fluorescence-Activated Cell Sortings,Microfluorimetry, Flow,Microfluorometries, Flow,Sorting, Fluorescence-Activated Cell,Sortings, Fluorescence-Activated Cell
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D001706 Biopsy Removal and pathologic examination of specimens from the living body. Biopsies
D013601 T-Lymphocytes Lymphocytes responsible for cell-mediated immunity. Two types have been identified - cytotoxic (T-LYMPHOCYTES, CYTOTOXIC) and helper T-lymphocytes (T-LYMPHOCYTES, HELPER-INDUCER). They are formed when lymphocytes circulate through the THYMUS GLAND and differentiate to thymocytes. When exposed to an antigen, they divide rapidly and produce large numbers of new T cells sensitized to that antigen. T Cell,T Lymphocyte,T-Cells,Thymus-Dependent Lymphocytes,Cell, T,Cells, T,Lymphocyte, T,Lymphocyte, Thymus-Dependent,Lymphocytes, T,Lymphocytes, Thymus-Dependent,T Cells,T Lymphocytes,T-Cell,T-Lymphocyte,Thymus Dependent Lymphocytes,Thymus-Dependent Lymphocyte
D015334 Gene Rearrangement, gamma-Chain T-Cell Antigen Receptor Ordered rearrangement of T-cell variable gene regions coding for the gamma-chain of antigen receptors. T-Cell Antigen Receptor gamma-Chain Gene Rearrangement,T-Lymphocyte Antigen Receptor gamma-Chain Gene Rearrangement,Gene Rearrangement, gamma-Chain T Cell Antigen Receptor,T Cell gamma-Chain Gene Rearrangement,T Lymphocyte gamma-Chain Gene Rearrangement,Gene Rearrangement, gamma Chain T Cell Antigen Receptor,T Cell Antigen Receptor gamma Chain Gene Rearrangement,T Cell gamma Chain Gene Rearrangement,T Lymphocyte Antigen Receptor gamma Chain Gene Rearrangement,T Lymphocyte gamma Chain Gene Rearrangement
D016207 Cytokines Non-antibody proteins secreted by inflammatory leukocytes and some non-leukocytic cells, that act as intercellular mediators. They differ from classical hormones in that they are produced by a number of tissue or cell types rather than by specialized glands. They generally act locally in a paracrine or autocrine rather than endocrine manner. Cytokine
D016692 Receptors, Antigen, T-Cell, gamma-delta T-cell receptors composed of CD3-associated gamma and delta polypeptide chains and expressed primarily in CD4-/CD8- T-cells. The receptors appear to be preferentially located in epithelial sites and probably play a role in the recognition of bacterial antigens. The T-cell receptor gamma/delta chains are separate and not related to the gamma and delta chains which are subunits of CD3 (see ANTIGENS, CD3). Antigen Receptors, T-Cell, gamma-delta,T-Cell Receptors delta-Chain,T-Cell Receptors gamma-Chain,T-Cell Receptors, gamma-delta,TcR gamma-delta,Antigen T Cell Receptor, delta Chain,Antigen T Cell Receptor, gamma Chain,Receptors, Antigen, T Cell, gamma delta,T Cell Receptors, gamma delta,T-Cell Receptor delta-Chain,T-Cell Receptor gamma-Chain,T-Cell Receptor, gamma-delta,T Cell Receptor delta Chain,T Cell Receptor gamma Chain,T Cell Receptor, gamma delta,T Cell Receptors delta Chain,T Cell Receptors gamma Chain,TcR gamma delta,delta-Chain, T-Cell Receptor,delta-Chain, T-Cell Receptors,gamma-Chain, T-Cell Receptor,gamma-Chain, T-Cell Receptors,gamma-delta T-Cell Receptor,gamma-delta T-Cell Receptors,gamma-delta, TcR
D019675 Genes, T-Cell Receptor gamma DNA sequences encoding the gamma chain of the T-cell receptor. The human gamma-chain locus is organized similarly to the TcR beta-chain locus. T-Cell Receptor gamma Genes,TcR gamma Genes,T-Cell Receptor gamma-Chain Genes,Gene, TcR gamma,Genes, T Cell Receptor gamma,Genes, TcR gamma,T Cell Receptor gamma Chain Genes,T Cell Receptor gamma Genes,TcR gamma Gene

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