Up-regulation of vaults may be necessary but not sufficient for multidrug resistance. 2001

A C Siva, and S Raval-Fernandes, and A G Stephen, and M J LaFemina, and R J Scheper, and V A Kickhoefer, and L H Rome
Department of Biological Chemistry, University of California at Los Angeles, School of Medicine, Los Angeles, CA 90095, USA.

Vaults are ribonucleoprotein complexes comprised of the 100 kDa major vault protein (MVP), the 2 high m.w. vault proteins p193 (VPARP) and p240 (TEP1) and an untranslated small RNA (vRNA). Increased levels of MVP, vault-associated vRNA and vaults have been linked directly to non-P-glycoprotein-mediated multidrug resistance (MDR). To further characterize the putative role of vaults in MDR, expression levels of all of the vault proteins were examined in various MDR cell lines. Subcellular fractionation of vault particles revealed that all 3 vault proteins are increased in MDR cells compared to the parental, drug-sensitive cells. Furthermore, protein analysis of subcellular fractions of the drug-sensitive, MVP-transfected AC16 cancer cell line indicated that vault levels are increased, in this stable line. Since TEP1 is shared by both vaults and the telomerase complex, TEP1 protein (and vault) levels were compared with telomerase activity in a variety of cell lines, including various MDR lines. Our studies demonstrate that while vault levels may be a good predictor of drug resistance, their up-regulation alone is not sufficient to confer the drug-resistant phenotype. This implies a requirement of an additional factor(s) for vault-mediated MDR.

UI MeSH Term Description Entries
D009369 Neoplasms New abnormal growth of tissue. Malignant neoplasms show a greater degree of anaplasia and have the properties of invasion and metastasis, compared to benign neoplasms. Benign Neoplasm,Cancer,Malignant Neoplasm,Tumor,Tumors,Benign Neoplasms,Malignancy,Malignant Neoplasms,Neoplasia,Neoplasm,Neoplasms, Benign,Cancers,Malignancies,Neoplasias,Neoplasm, Benign,Neoplasm, Malignant,Neoplasms, Malignant
D002352 Carrier Proteins Proteins that bind or transport specific substances in the blood, within the cell, or across cell membranes. Binding Proteins,Carrier Protein,Transport Protein,Transport Proteins,Binding Protein,Protein, Carrier,Proteins, Carrier
D005260 Female Females
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D012333 RNA, Messenger RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm. Messenger RNA,Messenger RNA, Polyadenylated,Poly(A) Tail,Poly(A)+ RNA,Poly(A)+ mRNA,RNA, Messenger, Polyadenylated,RNA, Polyadenylated,mRNA,mRNA, Non-Polyadenylated,mRNA, Polyadenylated,Non-Polyadenylated mRNA,Poly(A) RNA,Polyadenylated mRNA,Non Polyadenylated mRNA,Polyadenylated Messenger RNA,Polyadenylated RNA,RNA, Polyadenylated Messenger,mRNA, Non Polyadenylated
D014162 Transfection The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES. Transfections
D014407 Tumor Cells, Cultured Cells grown in vitro from neoplastic tissue. If they can be established as a TUMOR CELL LINE, they can be propagated in cell culture indefinitely. Cultured Tumor Cells,Neoplastic Cells, Cultured,Cultured Neoplastic Cells,Cell, Cultured Neoplastic,Cell, Cultured Tumor,Cells, Cultured Neoplastic,Cells, Cultured Tumor,Cultured Neoplastic Cell,Cultured Tumor Cell,Neoplastic Cell, Cultured,Tumor Cell, Cultured
D015854 Up-Regulation A positive regulatory effect on physiological processes at the molecular, cellular, or systemic level. At the molecular level, the major regulatory sites include membrane receptors, genes (GENE EXPRESSION REGULATION), mRNAs (RNA, MESSENGER), and proteins. Receptor Up-Regulation,Upregulation,Up-Regulation (Physiology),Up Regulation
D015972 Gene Expression Regulation, Neoplastic Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action in neoplastic tissue. Neoplastic Gene Expression Regulation,Regulation of Gene Expression, Neoplastic,Regulation, Gene Expression, Neoplastic
D016601 RNA-Binding Proteins Proteins that bind to RNA molecules. Included here are RIBONUCLEOPROTEINS and other proteins whose function is to bind specifically to RNA. Double-Stranded RNA-Binding Protein,Double-Stranded RNA-Binding Proteins,ds RNA-Binding Protein,RNA-Binding Protein,ds RNA-Binding Proteins,Double Stranded RNA Binding Protein,Double Stranded RNA Binding Proteins,Protein, Double-Stranded RNA-Binding,Protein, ds RNA-Binding,RNA Binding Protein,RNA Binding Proteins,RNA-Binding Protein, Double-Stranded,RNA-Binding Protein, ds,RNA-Binding Proteins, Double-Stranded,ds RNA Binding Protein

Related Publications

A C Siva, and S Raval-Fernandes, and A G Stephen, and M J LaFemina, and R J Scheper, and V A Kickhoefer, and L H Rome
September 2020, Kidney international reports,
A C Siva, and S Raval-Fernandes, and A G Stephen, and M J LaFemina, and R J Scheper, and V A Kickhoefer, and L H Rome
May 2006, The Journal of investigative dermatology,
A C Siva, and S Raval-Fernandes, and A G Stephen, and M J LaFemina, and R J Scheper, and V A Kickhoefer, and L H Rome
March 1993, Genes & development,
A C Siva, and S Raval-Fernandes, and A G Stephen, and M J LaFemina, and R J Scheper, and V A Kickhoefer, and L H Rome
January 2016, Virology,
A C Siva, and S Raval-Fernandes, and A G Stephen, and M J LaFemina, and R J Scheper, and V A Kickhoefer, and L H Rome
January 2019, Epidemiologia e prevenzione,
A C Siva, and S Raval-Fernandes, and A G Stephen, and M J LaFemina, and R J Scheper, and V A Kickhoefer, and L H Rome
October 1994, Journal of virology,
A C Siva, and S Raval-Fernandes, and A G Stephen, and M J LaFemina, and R J Scheper, and V A Kickhoefer, and L H Rome
September 2013, Academic medicine : journal of the Association of American Medical Colleges,
A C Siva, and S Raval-Fernandes, and A G Stephen, and M J LaFemina, and R J Scheper, and V A Kickhoefer, and L H Rome
January 2000, Psychological research,
A C Siva, and S Raval-Fernandes, and A G Stephen, and M J LaFemina, and R J Scheper, and V A Kickhoefer, and L H Rome
February 2015, Catheterization and cardiovascular interventions : official journal of the Society for Cardiac Angiography & Interventions,
A C Siva, and S Raval-Fernandes, and A G Stephen, and M J LaFemina, and R J Scheper, and V A Kickhoefer, and L H Rome
March 2019, The Australian and New Zealand journal of psychiatry,
Copied contents to your clipboard!