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We describe a fluorometric method for determining delta-aminolaevulinate dehydratase (EC 4.2.1.24) activity in human erythrocytes and compare it with the existing colorimetric method. Incubation conditions are identical. In the proposed method, the porphobilinogen formed during incubation is converted to a stable and highly fluorescent uroporphyrin by heating for 20 min at 93 degrees C in an acidic medium in the presence of air. The correlation between results by the two methods was good (r = 0.89). We found a good negative correlation between the activity of the enzyme and the concentration of circulating lead with both methods (r = -0.61 for present method and -0.59 for colorimetric method) for groups of persons subjected to various degrees of lead exposure. Our method is more sensitive and accurate, requires less sample, and the fluorescence is more stable than is the color in the colorimetric method.
S K Chakrabarti, and
J Brodeur, and
R Tardif
October 1974,
Clinical chemistry,
S K Chakrabarti, and
J Brodeur, and
R Tardif
January 1977,
Clinical chemistry,
S K Chakrabarti, and
J Brodeur, and
R Tardif
April 1974,
Analytical biochemistry,
S K Chakrabarti, and
J Brodeur, and
R Tardif
October 1997,
Pharmacology & toxicology,
S K Chakrabarti, and
J Brodeur, and
R Tardif
March 1974,
Archives of environmental health,
S K Chakrabarti, and
J Brodeur, and
R Tardif
January 1978,
Biochemical Society transactions,
S K Chakrabarti, and
J Brodeur, and
R Tardif
November 1966,
La Medicina del lavoro,
S K Chakrabarti, and
J Brodeur, and
R Tardif
May 1975,
The Journal of laboratory and clinical medicine,
S K Chakrabarti, and
J Brodeur, and
R Tardif
May 1985,
The Biochemical journal,
S K Chakrabarti, and
J Brodeur, and
R Tardif
August 1985,
The Biochemical journal,
