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Ficoll gradients have been used to enrich for heterokaryons in cultures of human skin fibroblasts following polyethylene glycol (PEG) induced fusion. These gradients provide a simple and consistent method for obtaining populations of multinucleated cells, at least twofold greater than those resulting from fusion alone. Formation of glucose-6-phosphate dehydrogenase (G6PD) heteropolymers has been used as a functional assay for the presence of heterokaryons. Analysis of cell populations enriched for multinucleated cells has revealed complementation leading to iduronate sulfatase activity in heterokaryons derived from iduronate sulfatase-deficient fibroblasts expressing the Hunter and multiple sulfatase-deficiency mutations.
L R Eisenberg, and
B R Migeon
January 1982,
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L R Eisenberg, and
B R Migeon
May 1985,
Biochimica et biophysica acta,
L R Eisenberg, and
B R Migeon
January 2016,
The open microbiology journal,
L R Eisenberg, and
B R Migeon
December 1979,
Proceedings of the National Academy of Sciences of the United States of America,
L R Eisenberg, and
B R Migeon
September 1978,
Bollettino della Societa italiana di biologia sperimentale,
L R Eisenberg, and
B R Migeon
July 2000,
Molecular genetics and metabolism,
L R Eisenberg, and
B R Migeon
May 1997,
Gene therapy,
L R Eisenberg, and
B R Migeon
November 1979,
Somatic cell genetics,
L R Eisenberg, and
B R Migeon
February 1982,
Journal of biochemistry,
L R Eisenberg, and
B R Migeon
September 1993,
Genomics,
