Identification of Dp71 isoforms in the platelet membrane cytoskeleton. Potential role in thrombin-mediated platelet adhesion. 2002

Richard C Austin, and Joan E B Fox, and Geoff H Werstuck, and Alan R Stafford, and Dennis E Bulman, and Ghassan Y Dally, and Cameron A Ackerley, and Jeffrey I Weitz, and Peter N Ray
Department of Pathology, McMaster University and the Henderson Research Centre, Hamilton, Ontario L8V 1C3, Canada. raustin@thrombosis.hhscr.org

Utrophin is a component of the platelet membrane cytoskeleton and participates in cytoskeletal reorganization (Earnest, J. P., Santos, G. F., Zuerbig, S., and Fox, J. E. B. (1995) J. Biol. Chem. 270, 27259-27265). Although platelets do not contain dystrophin, the identification of smaller C-terminal isoforms of dystrophin, including Dp71, which are expressed in a wide range of nonmuscle tissues and cell lines, has not been investigated. In this report, we have identified Dp71 protein variants of 55-60 kDa (designated Dp71Delta(110)) in the membrane cytoskeleton of human platelets. Both Dp71Delta(110) and utrophin sediment from lysed platelets along with the high speed detergent-insoluble pellet, which contains components of the membrane cytoskeleton. Like the membrane cytoskeletal proteins vinculin and spectrin, Dp71Delta(110) and utrophin redistributed from the high speed detergent-insoluble pellet to the integrin-rich low speed pellet of thrombin-stimulated platelets. Immunoelectron microscopy provided further evidence that Dp71Delta(110) was localized to the submembranous cytoskeleton. In addition to Dp71Delta(110), platelets contained several components of the dystrophin-associated protein complex, including beta-dystroglycan and syntrophin. To better understand the potential function of Dp71Delta(110), collagen adhesion assays were performed on platelets isolated from wild-type or Dp71-deficient (mdx(3cv)) mice. Adhesion to collagen in response to thrombin was significantly decreased in platelets isolated from mdx(3cv) mice, compared with wild-type platelets. Collectively, our results provide evidence that Dp71Delta(110) is a component of the platelet membrane cytoskeleton, is involved in cytoskeletal reorganization and/or signaling, and plays a role in thrombin-mediated platelet adhesion.

UI MeSH Term Description Entries
D008565 Membrane Proteins Proteins which are found in membranes including cellular and intracellular membranes. They consist of two types, peripheral and integral proteins. They include most membrane-associated enzymes, antigenic proteins, transport proteins, and drug, hormone, and lectin receptors. Cell Membrane Protein,Cell Membrane Proteins,Cell Surface Protein,Cell Surface Proteins,Integral Membrane Proteins,Membrane-Associated Protein,Surface Protein,Surface Proteins,Integral Membrane Protein,Membrane Protein,Membrane-Associated Proteins,Membrane Associated Protein,Membrane Associated Proteins,Membrane Protein, Cell,Membrane Protein, Integral,Membrane Proteins, Integral,Protein, Cell Membrane,Protein, Cell Surface,Protein, Integral Membrane,Protein, Membrane,Protein, Membrane-Associated,Protein, Surface,Proteins, Cell Membrane,Proteins, Cell Surface,Proteins, Integral Membrane,Proteins, Membrane,Proteins, Membrane-Associated,Proteins, Surface,Surface Protein, Cell
D008854 Microscopy, Electron Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen. Electron Microscopy
D008855 Microscopy, Electron, Scanning Microscopy in which the object is examined directly by an electron beam scanning the specimen point-by-point. The image is constructed by detecting the products of specimen interactions that are projected above the plane of the sample, such as backscattered electrons. Although SCANNING TRANSMISSION ELECTRON MICROSCOPY also scans the specimen point by point with the electron beam, the image is constructed by detecting the electrons, or their interaction products that are transmitted through the sample plane, so that is a form of TRANSMISSION ELECTRON MICROSCOPY. Scanning Electron Microscopy,Electron Scanning Microscopy,Electron Microscopies, Scanning,Electron Microscopy, Scanning,Electron Scanning Microscopies,Microscopies, Electron Scanning,Microscopies, Scanning Electron,Microscopy, Electron Scanning,Microscopy, Scanning Electron,Scanning Electron Microscopies,Scanning Microscopies, Electron,Scanning Microscopy, Electron
D009132 Muscles Contractile tissue that produces movement in animals. Muscle Tissue,Muscle,Muscle Tissues,Tissue, Muscle,Tissues, Muscle
D001792 Blood Platelets Non-nucleated disk-shaped cells formed in the megakaryocyte and found in the blood of all mammals. They are mainly involved in blood coagulation. Platelets,Thrombocytes,Blood Platelet,Platelet,Platelet, Blood,Platelets, Blood,Thrombocyte
D002448 Cell Adhesion Adherence of cells to surfaces or to other cells. Adhesion, Cell,Adhesions, Cell,Cell Adhesions
D002460 Cell Line Established cell cultures that have the potential to propagate indefinitely. Cell Lines,Line, Cell,Lines, Cell
D002462 Cell Membrane The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells. Plasma Membrane,Cytoplasmic Membrane,Cell Membranes,Cytoplasmic Membranes,Membrane, Cell,Membrane, Cytoplasmic,Membrane, Plasma,Membranes, Cell,Membranes, Cytoplasmic,Membranes, Plasma,Plasma Membranes
D003094 Collagen A polypeptide substance comprising about one third of the total protein in mammalian organisms. It is the main constituent of SKIN; CONNECTIVE TISSUE; and the organic substance of bones (BONE AND BONES) and teeth (TOOTH). Avicon,Avitene,Collagen Felt,Collagen Fleece,Collagenfleece,Collastat,Dermodress,Microfibril Collagen Hemostat,Pangen,Zyderm,alpha-Collagen,Collagen Hemostat, Microfibril,alpha Collagen
D003598 Cytoskeletal Proteins Major constituent of the cytoskeleton found in the cytoplasm of eukaryotic cells. They form a flexible framework for the cell, provide attachment points for organelles and formed bodies, and make communication between parts of the cell possible. Proteins, Cytoskeletal

Related Publications

Richard C Austin, and Joan E B Fox, and Geoff H Werstuck, and Alan R Stafford, and Dennis E Bulman, and Ghassan Y Dally, and Cameron A Ackerley, and Jeffrey I Weitz, and Peter N Ray
November 1987, Journal of the National Cancer Institute,
Richard C Austin, and Joan E B Fox, and Geoff H Werstuck, and Alan R Stafford, and Dennis E Bulman, and Ghassan Y Dally, and Cameron A Ackerley, and Jeffrey I Weitz, and Peter N Ray
July 1988, Cancer research,
Richard C Austin, and Joan E B Fox, and Geoff H Werstuck, and Alan R Stafford, and Dennis E Bulman, and Ghassan Y Dally, and Cameron A Ackerley, and Jeffrey I Weitz, and Peter N Ray
January 1985, Biochemical and biophysical research communications,
Richard C Austin, and Joan E B Fox, and Geoff H Werstuck, and Alan R Stafford, and Dennis E Bulman, and Ghassan Y Dally, and Cameron A Ackerley, and Jeffrey I Weitz, and Peter N Ray
October 2013, Archives of biochemistry and biophysics,
Richard C Austin, and Joan E B Fox, and Geoff H Werstuck, and Alan R Stafford, and Dennis E Bulman, and Ghassan Y Dally, and Cameron A Ackerley, and Jeffrey I Weitz, and Peter N Ray
December 1995, Journal of neuro-oncology,
Richard C Austin, and Joan E B Fox, and Geoff H Werstuck, and Alan R Stafford, and Dennis E Bulman, and Ghassan Y Dally, and Cameron A Ackerley, and Jeffrey I Weitz, and Peter N Ray
January 1986, Agents and actions. Supplements,
Richard C Austin, and Joan E B Fox, and Geoff H Werstuck, and Alan R Stafford, and Dennis E Bulman, and Ghassan Y Dally, and Cameron A Ackerley, and Jeffrey I Weitz, and Peter N Ray
October 1984, The Journal of laboratory and clinical medicine,
Richard C Austin, and Joan E B Fox, and Geoff H Werstuck, and Alan R Stafford, and Dennis E Bulman, and Ghassan Y Dally, and Cameron A Ackerley, and Jeffrey I Weitz, and Peter N Ray
June 1978, Science (New York, N.Y.),
Richard C Austin, and Joan E B Fox, and Geoff H Werstuck, and Alan R Stafford, and Dennis E Bulman, and Ghassan Y Dally, and Cameron A Ackerley, and Jeffrey I Weitz, and Peter N Ray
November 1995, Experimental cell research,
Richard C Austin, and Joan E B Fox, and Geoff H Werstuck, and Alan R Stafford, and Dennis E Bulman, and Ghassan Y Dally, and Cameron A Ackerley, and Jeffrey I Weitz, and Peter N Ray
May 1981, Thrombosis research,
Copied contents to your clipboard!