Anti-idiotypic antibody specific for an infectious bursal disease virus-neutralizing monoclonal antibody does not interfere with the virus infection. 2002

T Yamaguchi, and A Igusa, and A Setiyono, and H Fukushi, and K Hirai
Department of Veterinary Microbiology, Faculty of Agriculture, Gifu University, Japan. yamaguti@cc.gifu-u.ac.jp

Infectious bursal disease virus (IBDV) capsid protein, VP2, contains a hypervariable domain that is recognized by virus-neutralizing antibodies. The virus-neutralizing epitope is highly conformation-dependent and the domain is speculated to be involved in the virus-target cell interaction. In this study, a polyclonal anti-idiotypic antibody (anti-id) was generated by the sequential immunization of a rabbit with a virus-neutralizing monoclonal antibody GI-11 which recognizes the VP2 hypervariable domain. Although the anti-id, which mimics the conformational epitope in the VP2 hypervariable domain, was expected to inhibit the virus infection, the anti-id did not interfere with either the virus binding or the infection to the target cell.

UI MeSH Term Description Entries
D007243 Infectious bursal disease virus A species of AVIBIRNAVIRUS causing severe inflammation of the bursa of Fabricius in chickens and other fowl. Transmission is thought to be through contaminated feed or water. Vaccines have been used with varying degrees of success. Avian Nephrosis Virus,Gumboro Disease Virus,IBDV,Infectious Bursal Agent,Bursal Agent, Infectious,Bursal Disease Virus, Infectious,Avian Nephrosis Viruses,Bursal Agents, Infectious,Infectious Bursal Agents,Nephrosis Virus, Avian,Nephrosis Viruses, Avian
D009500 Neutralization Tests The measurement of infection-blocking titer of ANTISERA by testing a series of dilutions for a given virus-antiserum interaction end-point, which is generally the dilution at which tissue cultures inoculated with the serum-virus mixtures demonstrate cytopathology (CPE) or the dilution at which 50% of test animals injected with serum-virus mixtures show infectivity (ID50) or die (LD50). Neutralization Test,Test, Neutralization,Tests, Neutralization
D011817 Rabbits A burrowing plant-eating mammal with hind limbs that are longer than its fore limbs. It belongs to the family Leporidae of the order Lagomorpha, and in contrast to hares, possesses 22 instead of 24 pairs of chromosomes. Belgian Hare,New Zealand Rabbit,New Zealand Rabbits,New Zealand White Rabbit,Rabbit,Rabbit, Domestic,Chinchilla Rabbits,NZW Rabbits,New Zealand White Rabbits,Oryctolagus cuniculus,Chinchilla Rabbit,Domestic Rabbit,Domestic Rabbits,Hare, Belgian,NZW Rabbit,Rabbit, Chinchilla,Rabbit, NZW,Rabbit, New Zealand,Rabbits, Chinchilla,Rabbits, Domestic,Rabbits, NZW,Rabbits, New Zealand,Zealand Rabbit, New,Zealand Rabbits, New,cuniculus, Oryctolagus
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D000888 Antibodies, Anti-Idiotypic Antibodies which react with the individual structural determinants (idiotopes) on the variable region of other antibodies. Anti-Antibodies,Anti-Idiotype Antibodies,Antibodies, Internal Image,Antigamma Globulin Antibodies,Antiglobulins,Anti Antibodies,Anti-gamma Globulin Antibodies,Anti Idiotype Antibodies,Anti gamma Globulin Antibodies,Anti-Idiotypic Antibodies,Antibodies, Anti,Antibodies, Anti Idiotypic,Antibodies, Anti-Idiotype,Antibodies, Anti-gamma Globulin,Antibodies, Antigamma Globulin,Globulin Antibodies, Anti-gamma,Globulin Antibodies, Antigamma,Image Antibodies, Internal,Internal Image Antibodies
D000911 Antibodies, Monoclonal Antibodies produced by a single clone of cells. Monoclonal Antibodies,Monoclonal Antibody,Antibody, Monoclonal
D000914 Antibodies, Viral Immunoglobulins produced in response to VIRAL ANTIGENS. Viral Antibodies
D015678 Viral Structural Proteins Viral proteins that are components of the mature assembled VIRUS PARTICLES. They may include nucleocapsid core proteins (gag proteins), enzymes packaged within the virus particle (pol proteins), and membrane components (env proteins). These do not include the proteins encoded in the VIRAL GENOME that are produced in infected cells but which are not packaged in the mature virus particle,i.e. the so called non-structural proteins (VIRAL NONSTRUCTURAL PROTEINS). Polypeptide VP1, Structural,VP(1),VP(2),VP(3),VP(6),VP(7),Viral Structural Proteins VP,Virus Structural Proteins,Proteins, Viral Structural,Proteins, Virus Structural,Structural Polypeptide VP1,Structural Proteins, Viral,Structural Proteins, Virus,VP1, Structural Polypeptide
D018175 Birnaviridae Infections Virus diseases caused by the BIRNAVIRIDAE. Infections, Birnaviridae,Birnaviridae Infection,Infection, Birnaviridae

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