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A method is described for the preparation of high purity myosin from small amounts of cardiac muscle. The method employs homogenization and prolonged extraction of the cardiac tissue. Purification is achieved through three successive precipitation-dissolution cycles and without the use of column chromatographic techniques. Purity of the myosin preparation is assessed at various stages of the purification procedure by sodium dodecylsulfate-acrylamide gel electrophoresis and by measurement of RNA and nucleoprotein content. With 1.5-2.0 g of rabbit right ventricle as the starting tissue, this method yields 4-6 mg myosin per g wet tissue. The method is also shown to give similar results with rabbit right ventricles hypertrophied by pulmonary stenosis.
K T Shiverick, and
L L Thomas, and
N R Alpert
July 1983,
Circulation research,
K T Shiverick, and
L L Thomas, and
N R Alpert
November 2004,
British journal of pharmacology,
K T Shiverick, and
L L Thomas, and
N R Alpert
April 1977,
Biochimica et biophysica acta,
K T Shiverick, and
L L Thomas, and
N R Alpert
January 1982,
Virchows Archiv. A, Pathological anatomy and histology,
K T Shiverick, and
L L Thomas, and
N R Alpert
April 1994,
Proceedings of the National Academy of Sciences of the United States of America,
K T Shiverick, and
L L Thomas, and
N R Alpert
July 1973,
Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.),
K T Shiverick, and
L L Thomas, and
N R Alpert
September 1999,
Journal of cellular biochemistry,
K T Shiverick, and
L L Thomas, and
N R Alpert
January 1995,
Chinese medical journal,
K T Shiverick, and
L L Thomas, and
N R Alpert
October 1979,
Klinicheskaia meditsina,
K T Shiverick, and
L L Thomas, and
N R Alpert
October 2000,
Heart failure reviews,
