Biomaterial Database

[Determination of zopiclone in serum by reversed-phase high performance liquid chromatography]. 2002

Li-jun Yang, and Gertrud Rochholz

Department of Narcotics Control, Yunnan Public Security College, Kunming 650223, China. ylj666@public.km.yn.cn

A reversed-phase high performance liquid chromatographic method (external standard method) was developed for the determination of zopiclone in serum. After the selective extraction with n-butyl chloride, this compound was chromatographed on a LiChroCART 125-4 column packed with LiChrospher 60 RP select B(5 microns) using acetonitrile-monopotassium phosphate (20:80, V/V) as mobile phase. The eluting compound was measured by an ultraviolet detector at 254 nm. Spiked with zopiclone of 400 micrograms/L, 1,600 micrograms/L and 6,400 micrograms/L in serum, the recoveries of zopiclone were (73.4 +/- 3.2)%, (82.2 +/- 4.1)% and (90.3 +/- 4.5)% respectively. The detection limit of the method was 15 micrograms/L. The method is simple and rapid for the determination of zopiclone in forensic toxicology.

UI	MeSH Term	Description	Entries
D006993	Hypnotics and Sedatives	Drugs used to induce drowsiness or sleep or to reduce psychological excitement or anxiety.	Hypnotic,Sedative,Sedative and Hypnotic,Sedatives,Hypnotic Effect,Hypnotic Effects,Hypnotics,Sedative Effect,Sedative Effects,Sedatives and Hypnotics,Effect, Hypnotic,Effect, Sedative,Effects, Hypnotic,Effects, Sedative,Hypnotic and Sedative
D008297	Male		Males
D010879	Piperazines	Compounds that are derived from PIPERAZINE.
D002110	Caffeine	A methylxanthine naturally occurring in some beverages and also used as a pharmacological agent. Caffeine's most notable pharmacological effect is as a central nervous system stimulant, increasing alertness and producing agitation. It also relaxes SMOOTH MUSCLE, stimulates CARDIAC MUSCLE, stimulates DIURESIS, and appears to be useful in the treatment of some types of headache. Several cellular actions of caffeine have been observed, but it is not entirely clear how each contributes to its pharmacological profile. Among the most important are inhibition of cyclic nucleotide PHOSPHODIESTERASES, antagonism of ADENOSINE RECEPTORS, and modulation of intracellular calcium handling.	1,3,7-Trimethylxanthine,Caffedrine,Coffeinum N,Coffeinum Purrum,Dexitac,Durvitan,No Doz,Percoffedrinol N,Percutaféine,Quick-Pep,Vivarin,Quick Pep,QuickPep
D002851	Chromatography, High Pressure Liquid	Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed.	Chromatography, High Performance Liquid,Chromatography, High Speed Liquid,Chromatography, Liquid, High Pressure,HPLC,High Performance Liquid Chromatography,High-Performance Liquid Chromatography,UPLC,Ultra Performance Liquid Chromatography,Chromatography, High-Performance Liquid,High-Performance Liquid Chromatographies,Liquid Chromatography, High-Performance
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000368	Aged	A person 65 years of age or older. For a person older than 79 years, AGED, 80 AND OVER is available.	Elderly
D053961	Azabicyclo Compounds	Bicyclic bridged compounds that contain a nitrogen which has three bonds. The nomenclature indicates the number of atoms in each path around the rings, such as [2.2.2] for three equal length paths. Some members are TROPANES and BETA LACTAMS.	Azabicyclo(1.1.0)Butanes,Azabicyclo(2.2.2)Octanes,Azabicyclo(3.3.1)Nonanes,Azabicyclo(4.3.0)Nonanes,Azabicyclo(5.2.2)Undecanes